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Genetic engineering /

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Bibliographic Details
Main Author: Williams, J. G. (Jeff G.)
Other Authors: Ceccarelli, A., Wallace, A. (Andrew), 1963-
Format: Book
Language:English
Published: Oxford : New York : BIOS Scientific Publishers ; Springer-Verlag, 2001.
Edition:Second edition.
Series:Medical perspectives series.
Subjects:
Chromosome Mapping.
Cloning, Molecular.
Gene Expression.
Genetic Engineering.
Table of Contents:
  • Gene organization and expression
  • General principles of gene organization and replication
  • Common features of prokaryotic and eukaryotic gene expression
  • Characteristic features of prokaryotic gene organization and expression
  • Eukaryotic mRNA structure and post-transcriptional regulation
  • Eukaryotic gene transcription and its regulation
  • Transcriptional termination and processing
  • Splicing
  • Chromosome structure
  • References
  • Further reading
  • Gene analysis techniques I: low-resolution mapping of genomic DNA
  • Nucleic acid hybridization
  • Gel electrophoresis and detection of nucleic acids by Southern and Northern blotting
  • Mapping genes to chromosomes
  • Restriction enzyme mapping and its applications
  • References
  • Further reading
  • Gene analysis techniques II: determining the structure of genes
  • DNA sequence analysis
  • Transcript mapping techniques
  • Searching for genes using computers
  • The polymerase chain reaction
  • References
  • Further reading
  • Application of PCR technology to human diseases
  • Genotyping using PCR technology
  • Molecular analysis of clinical samples
  • Diagnosing infectious diseases by PCR
  • References
  • Further reading
  • Cloning in E. coli I: vectors and genomic libraries
  • Cloning and cloning vectors
  • Plasmid vectors and their use in manipulating DNA
  • Phage vectors
  • Construction and screening of genomic libraries
  • References
  • Further reading
  • Cloning in E. coli II: isolating genes
  • Construction of cDNA libraries
  • Screening cDNA libraries
  • Identifying genes by positional cloning: cystic fibrosis
  • References
  • Further reading
  • Cloning in higher organisms
  • Cloning in yeast
  • Transformation of mammalian cells in tissue culture
  • The creation and use of transgenic mice
  • The impact of whole genome DNA sequence determination: proteomics and micro-arrays.