Nuclease methods and protocols /

Bibliographic Details
Other Authors: Schein, Catherine H.
Format: Book
Language:English
Published: Totowa, NJ : Humana Press, [2001]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 160.
Subjects:
Table of Contents:
  • Processivity of DNA repair enzymes / R.S. Lloyd
  • Analysis of HPLC of distributive activities and the synthetic (back) reaction of pancreatic-type ribonucleases / M.V. Nogués ... [et al.]
  • Ire1p : a kinase and site-specific endoribonuclease / T.N. Gonzalez ... [et al.]
  • Microtiter-plate assay and related assays for nonspecific endonucleases / G. Meiss ... [et al.]
  • Quantitating mRNAs with relative and competitive RT-PCR / E.A. Prediger
  • Expressing self-incompatibility RNases (S-RNases) in transgenic plants / B. Beecher ... [et al.]
  • Molecular cloning, tissue distribution, and chromosomal localization of the human homolog of the R2/Th/Stylar ribonuclease gene family / F. Acquati ... [et al.]
  • Ribonuclease inhibitors / B.L. Pasloske
  • Producing soluble recombinant RNases and assays to measure their interaction and interferon-delta in vitro / C.H. Schein
  • Assays for the evaluation of HIV-1 integrase inhibitors / Z. Debyser ... [et al.]
  • The direction of ribonucleases H by antisense oligodeoxynucleotides / R.V. Giles ... [et al.]
  • The 2-5A/RNase L pathway and inhibition by RNase L inhibitor (RLI) / C. Bisbal ... [et al.]
  • Crystallization and crystal structure determination of ribonuclease A-ribonuclease inhibitor protein complex / B. Kobe
  • Methods for studying the interaction of barnase with its inhibitor barstar / G. Schreiber
  • Assaying in vitro refolding of RNases by mass spectrometry / G. Marino ... [et al.]
  • Dissecting nucleases into their structural and functional domains : mapping the RNA-binding surface of RNase III by NMR / A. Ramos ... [et al.]
  • Using electrostatics to define the active site of Serratia endonuclease / K.L. Krause ... [et al.]
  • Homology modeling and simulations of nuclease structures / K.V. Soman ... [et al.]
  • Methods for determining activity and specificity of DNA binding and DNA cleavage by class II restriction endonucleases / A. Jeltsch ... [et al.]
  • Engineered properties and assays for human DNase I mutants / C.Q. Pan ... [et al.]
  • Assays for human DNase I activity in biological matrices / D.V. Sinicropi ... [et al.]
  • Evaluation of retroviral ribonuclease H activity / J.T. Miller ... [et al.]
  • Assays for detection of RNase A superfamily ribonucleases / H.F. Rosenberg ... [et al.]
  • Assay for antitumor and lectin activity of angiogenin / J.F. Riordan ... [et al.]
  • Preparation and preclinical characterization of RNase-based immunofusion proteins / D.L. Newton ... [et al.]
  • Restriction endonucleases and their uses / R.J. Williams
  • Isolation and characterization of an unknown restriction endonuclease / R.J. Williams
  • Gene modification with hapaxoterministic restriction enzymes : easing the way / S.L. Berger
  • Techniques to measure nucleic acid-protein binding and specificity : nuclear extract preparations, DNase I footprinting, and mobility shift assays / R.A. Rippe ... [et al.]
  • Analyzing the developmental expression of sigma factors with S1-nuclease mapping / J. Kormanec
  • Detection and quantitation of mRNAs using ribonuclease protection assays / E.A. Prediger