Interleukin protocols /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2001]
|
| Series: | Methods in molecular medicine ;
60. |
| Subjects: |
Table of Contents:
- Pt. I. General methods for assaying interleukin protin and mRNA
- ELISAs and interleukin research
- ELISPOT technique for assaying interleukins
- Measurement of interleukins by ELISPOT assay with particular application to dual-color analysis (stardust assay)
- Assaying interleukins intracellularly by FACS
- Basic RT-PCR for measurement of cytokine expression
- Quantitative PCR for measurement of cytokine expression
- Single cell RT-PCR for the detection of cytokine mRNA
- Quantitation of cytokine mRNA by flash-type bioluminescence
- Measurement of interleukin mRNA by Norther Blotting using a nonradioactive detection method
- Quantification of mouse IL-6 and TNF-alpha mRNA using Xplore assays
- Measurement of cytokine and chemokine mRNA using nonisotopic multiprobe RNase protection assay
- Isolation of total and bioactive interleukins by immunoaffinity-receptor affinity chromatography
- Detection of cytokines by immunohistochemistry
- Whole blood assays and the influence of circadian rhythmicity on human cytokine measurement
- Pt. II. Illustration of methodologies using individual interleukins
- Assaying interleukin-8
- Measurement of interleukine-2 by bioassay
- Assaying interleukin-15
- Pt. III. Assaying interleukins in particular pathologies
- Assaying interleukin-6 in breast cancer
- Cytokines, stress, and depressive illness
- Measurement of interleukins in cutaneous disorders : competitive RT-PCR for determination of IL-10 mRNA expression in psoriasis
- Interleukins in Graves' disease
- Evaluation of cytokines in migraine patients by ELISA
- Assaying interleukins in plasma in the course of acute myocardial infarction
- Pt. IV. Assaying interleukins in particular biological fluids
- Measurement of cytokines in peritoneal fluids
- Measurement of cytokines in seminal plasma
- Measurement of cytokines in induced sputum : application to the investigation of asthma and COPD
- Cytokines in blister fluids
- Measurement of cytokines in synovial fluid
- Measurement of cytokines in cerebrospinal fluid
- Detection of cytokines in tears
- Pt. V. Newer techniques : interleukin signal transduction, recombinant interleukin production, interleukin gene polymorphisms, and the use of cDNA arrays
- Measurement of phosphoinositide 3-kinase activation by interleukins
- Analysis of interleukin-2 signaling using affinity precipitations and polyacrylamide gel electrophoresis
- Applications for green fluorescent protein in cell signaling
- Use of Pichia pastoris for production of recombinant cytokines
- Analysis of single nucleotide polymorphisms in the interleukin-1B gene usint 5' nuclease assays
- Measurement of cytokine and chemokine gene expression patterns using cDNA array