Kinesin protocols /

Bibliographic Details
Other Authors: Vernos, Isabelle
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2001]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 164.
Subjects:
Table of Contents:
  • Purification of kinesin from the brain / S.A. Kuznetsov ... [et al.]
  • RT-PCR for the identification of developmentally regulated novel members of the kinesin-like superfamily / N. Santama
  • Expression cloning with pan kinesin antibodies / L.M. Ginkel ... [et al.]
  • Expression of kinesin in Escherichia coli / M.F. Stock ... [et al.]
  • Plasmids for expression of chimeric and truncated kinesin proteins / K.W. Waligora ... [et al.]
  • Preparation of recombinant kinesin superfamily proteins using the baculovirus system / N. Hirokawa ... [et al.]
  • Assays for kinesin microtubule-stimulated ATPase activity / D.D. Hackney ... [et al.]
  • An improved microscope for head and surface-based motility assays / N. Carter ... [et al.]
  • Use of photonic force microscopy to study single-motor-molecule mechanics / S. Jeney ... [et al.]
  • Assays for microtubule-destabilizing kinesins / A. Desai ... [et al.]
  • Green fluorescent protein as a tag for molecular motor proteins / S.A. Endow
  • In vitro reconstitution of endosome motility along microtubules / E. Nielsen ... [et al.]
  • Approaches to study interactions between kinesin motors and membranes / G. Morfini ... [et al.]
  • Microinjection methods for analyzing the functions of kinesins in early embryos / R.L. Morris ... [et al.]
  • The use of dominant negative mutants to study the function of mitotic motors in the in vitro spindle assembly assay in Xenopus egg extracts / H. Boleti ... [et al.]
  • A dominant negative approach for functional studies of the kinesin II complex / V.I. Gelfand ... [et al.]
  • Identification of kinesin-associated proteins / L.C. Lindesmith ... [et al.]
  • Assaying spatial organization of microtubules by kinesin motors / F. Nédélec ... [et al.]
  • Crystallization of kinesin / M. Thormählen ... [et al.]
  • Structural analysis of the microtubule-kinesin complex by cryo-electron microscopy / F. Beuron ... [et al.]