http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Vol. 1 -- Getting started in molecular biology / T.A. Brown -- Practical requirements for molecular biology research -- Health hazards and safety procedures -- Research strategies for molecular biology -- Planning an informative project -- Microbiological techniques for molecular biology: bacteria and phages / Brian W. Bainbridge -- Introduction: techniques for handling microbes -- Culturing of Escherichia coli -- Characterization of bacterial strains -- Preservation of stock cultures -- Culturing of bacteriophages and M13 -- Troubleshooting -- Purification of DNA / Paul Towner -- Silica-based methods for the isolation of DNA -- Assessment of quality -- Purification of RNA / Miles Wilkinson -- Ribonuclease-free conditions -- Quantification of RNA -- Precipitation and storage of RNA -- Preparation of total cellular RNA -- Cytoplasmic and nuclear RNA -- Poly(A)⁺RNA -- Nucleic acid electrophoresis in agarose gels / Douglas H. Robinson, Gayle J. Lafleche -- Preparation of agarose gels -- Vertical agarose gels and fast-running horizontal gels -- Electrophoresis of RNA -- Recovery of DNA from electrophoresis gels / Paul Towner -- Agarose gels -- Polyacrylamide gels -- Construction of recombinant DNA molecules / Richard Powell, Frank Gannon -- Restriction enzyme digestions -- Preparation of vectors for molecular cloning experiments -- Construction of recombinant molecules -- Generation and identification of recombinant clones / T.A. Brown -- Introduction of DNA into E. coli cells -- Plating out and recombinant selection -- Vectors combining features of both plasmids and phages -- Survey of cloning vectors for Escherichia coli / T.A. Brown -- E. coli as the host organism for recombinant DNA research -- Plasmid vectors -- Bacteriophage vectors -- Bacteriophage M13 vectors -- Appendices -- Important Escherichia coli strains -- Recipes and general procedures -- Restriction endonucleases -- DNA and RNA modification enzymes -- List of suppliers.
Vol. 2. -- Strategies for research in molecular biology -- Construction of genomic libraries and cosmid vectors -- Construction of a cDNA library -- Nucleic acid labelling and detection -- Immobilization of nucleic acids and hybridization analysis -- DNA sequencing -- Polymerase chain reaction -- Protein expression in Escherichia coli -- Transcript mapping
Includes bibliographical references and index.