rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Essential molecular biology
    sub title: a practical approach
  name:
    name part: Brown, T. A. (Terence A.)
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: enk
    date issued: 2000
    edition: Second edition.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: 2 volumes : illustrations ; 26 cm.
  table of contents: Vol. 1 -- Getting started in molecular biology / T.A. Brown -- Practical requirements for molecular biology research -- Health hazards and safety procedures -- Research strategies for molecular biology -- Planning an informative project -- Microbiological techniques for molecular biology: bacteria and phages / Brian W. Bainbridge -- Introduction: techniques for handling microbes -- Culturing of Escherichia coli -- Characterization of bacterial strains -- Preservation of stock cultures -- Culturing of bacteriophages and M13 -- Troubleshooting -- Purification of DNA / Paul Towner -- Silica-based methods for the isolation of DNA -- Assessment of quality -- Purification of RNA / Miles Wilkinson -- Ribonuclease-free conditions -- Quantification of RNA -- Precipitation and storage of RNA -- Preparation of total cellular RNA -- Cytoplasmic and nuclear RNA -- Poly(A)⁺RNA -- Nucleic acid electrophoresis in agarose gels / Douglas H. Robinson, Gayle J. Lafleche -- Preparation of agarose gels -- Vertical agarose gels and fast-running horizontal gels -- Electrophoresis of RNA -- Recovery of DNA from electrophoresis gels / Paul Towner -- Agarose gels -- Polyacrylamide gels -- Construction of recombinant DNA molecules / Richard Powell, Frank Gannon -- Restriction enzyme digestions -- Preparation of vectors for molecular cloning experiments -- Construction of recombinant molecules -- Generation and identification of recombinant clones / T.A. Brown -- Introduction of DNA into E. coli cells -- Plating out and recombinant selection -- Vectors combining features of both plasmids and phages -- Survey of cloning vectors for Escherichia coli / T.A. Brown -- E. coli as the host organism for recombinant DNA research -- Plasmid vectors -- Bacteriophage vectors -- Bacteriophage M13 vectors -- Appendices -- Important Escherichia coli strains -- Recipes and general procedures -- Restriction endonucleases -- DNA and RNA modification enzymes -- List of suppliers.
  table of contents: Vol. 2. -- Strategies for research in molecular biology -- Construction of genomic libraries and cosmid vectors -- Construction of a cDNA library -- Nucleic acid labelling and detection -- Immobilization of nucleic acids and hybridization analysis -- DNA sequencing -- Polymerase chain reaction -- Protein expression in Escherichia coli -- Transcript mapping
  note: edited by T.A. Brown.
  note: Includes bibliographical references and index.
  subject:
    topic: Cloning, Molecular
  subject:
    topic: DNA, Recombinant
  related item:
    title info:
      title: Practical approach series
  identifier: 0199636427 (pbk. : v. 1)
  identifier: 0199636435 (hard : v. 1)
  record info:
    record content source: SMC
    record creation date: 001205
    record change date: 20191203081900.0
    record identifier: in00004491903