Forensic DNA typing : biology & technology behind STR markers /
| Main Author: | |
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| Format: | Book |
| Language: | English |
| Published: |
Amsterdam ; Boston :
Academic Press,
[2001]
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| Subjects: |
Table of Contents:
- DNA biology review
- Sample collection and preparation (DNA extraction and quantification)
- The polymerase chain reaction (DNA amplification)
- Commonly used short tandem repeat markers
- Biology of STRs : stutter products, non-template addition, microvariants, null alleles, and mutation rates
- Forensic issues : degraded DNA, PCR inhibition, contamination, and mixed samples
- Additional DNA markers : amelogenin, Y-chromosome STRs, mtDNA, SNPs, Alu repeats
- DNA separation methods : slab-gel and capillary electrophoresis
- DNA detection methods : fluorescent dyes and silver staining
- The Abi prism 310 genetic analyzer
- The hitachi FMBIO II fluorescence imaging system
- STR genotyping issues
- Laboratory validation
- New technologies and automation
- Combined DNA index system (CODIS) and the use of DNA databases
- DNA testing in high profile cases