Rapid cycle real-time PCR : methods and applications /
| Other Authors: | , , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Berlin ; New York :
Springer,
[2001]
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| Subjects: |
Table of Contents:
- Mutation detection by fluorescent hybridization probe melting curves
- Quantification on the LightCycler
- Selection of hybridization probes for real-time quantification and genetic analysis
- Using the nearest neighbor model for the estimation of matched and mismatched hybridization probe melting points and selection of optimal probes on the LightCycler
- Quantification of human papilloma virus type 16 using quantitative competitive PCR on the LightCycler
- Use of TaqStart antibody to increase the sensitivity of herpesvirus quantitative PCR on the LightCycler
- High-speed detection of the alpha₁-antitrypsin deficiency alleles Pi*S and Pi*Z on the LightCycler
- High-speed methylenetetrahydrofolate reductase C{right arrow] T677 mutation detection on the LightCycler
- Dual color detection of splice variants of the c-erA alpha (thyroid hormone receptor alpha) gene
- Detection of three major polymorphisms in the N-acetyltransferase 2 gene by melting peak analysis using fluorogenic hybridization probes
- Genotyping of cytochrome P450 2D6*4 mutation with fluorescent hybridization probes using LightCycler
- Fluorescent hybridization probe detection of the F508del cystic fibrosis allele on the LightCycler
- Genotyping beta-globin mutations (Hb S, Hb C, Hb E) by multiplexing probe color and melting temperature
- Simultaneous detection of C282Y and H63D hemochromatosis mutations using LCRed 640 and LCRed 705 labeled hybridization probes
- Genotyping of angiotensin-converting enzyme and angiotensinogen polymorphisms with the LightCycler
- Genotyping of the most common thiopurine methyltransferase mutations with the LightCycler
- Detection of the mitochondrial DNA mutation MELAS3243 using hybridization probes
- Detection of the p53 allele deletions in human cancer by quantification of genomic copy number
- Monitoring of residual disease in patients with chronic myelogenous leukemia using specific fluorescent hybridization probes for real-time quantitative RT-PCR
- Development of quantitative RT-PCR for the expression of Wilms' tumor WT1 suppressor gene in leukemia on the LightCycler
- Real-time detection of minimal residual disease by amplifying immunoglobulin genes in acute lymphoblastic leukemia on the LightCycler
- NER2/neu gene amplification quantified by PCR and melting peak analysis using a single base alteration competitor as an internal standard
- Quantification of residual tumor cells in monoclonal B-cell lymphoma
- Development of PCR-based assays for the detection of chromosomal translocations using SYBR green I
- Relative quantification of the HER1/neu oncogene using SYBR green I.