Lipase and phospholipase protocols /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1999]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 109. |
| Subjects: |
Table of Contents:
- Phospholipase A2 and phosphatidylinositol-specific phospholipase C assays by HPLC and TLC with fluorescent substrate
- Fluorometric phospholipase assays based on polymerized lipsome substrates
- Triglyceride lipase assays based on a novel fluorogenic alkyldiacyl glycerol substrate
- Purifaction and assay of mammalian group I and group IIa secretory phospholipase A₂
- Determination of plasmalogen-selective phospholipase A₂ activity by radiochemical and fluorometric assay procedures
- Human plasma platelet-activating factor acetylhydrolase
- Assays for pancreatic triglyceride lipase and colipase
- Bile salt-activated lipase
- Determining lipoprotein lipase and hepatic lipase activity using radiolabeled substrates
- Lysosomal acid lipase : assay and purification
- Hormone-sensitive lipase and neutral cholesterol
- Lecithin-cholesterol acyltransferase : assay of cholesterol esterification and phospholipase A₂ activities
- Purification of lipases and phospholipases by Heparin-Sepharose chromatography
- Large scale lipoprotein lipase purification from adipose tissue
- Purification of rat hepatic lipase essentially free of apolipoprotein E and apolipoprotein B
- High-level expression and purification of human hepatic lipase from mammalian cells
- High-level baculoviral expression of hormone-sensitive lipase
- High-level baculoviral expression of lysosomal acid lipase
- One-step purification and biochemical characterization of recombinant pancreatic lipases expressed in insect cells
- Modulation of the expression level of human acidic lapases by various signal peptides
- Immunodetection of lipoprotein lipase : antibody production, immunoprecipitation, and western blotting techniques
- Immumological characterization of digestive lipases
- Determining lipase subunit structure by sucrose gradient centrifugation
- Detecting ligands interacting with lipoprotein lipase
- Monolayer techniques for studying lipase kinetics
- Efficient immobilization of phospholipase A₂
- An introduction to internet resources for the molecular and genetic analysis of the lipases
- Techniques for the measurement of lipoprotein lipase messenger RNA
- In Vitro transcription and translation of lipoprotein lipase
- Induced lipase mutations in the mouse