http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Cytochrome P four hundred fifty protocols
The role of methods in the discovery of the cytochrome P450 -- Cytochrome P450 nomenclature -- Spectral analyses of cytochrome P450 -- Purification of cytochromes P450 : rat and human hepatic forms -- Isolation and purification of constitutive forms of microsomal cytochrome P450 -- Purification of extrahepatic cytochromes P450 -- Purification of cytochromes P450 : products of bacterial recombinant expression systems -- Cytochrome P450 reconstitution systems -- Catalytic assays for human cytochrome P450 -- Enzymatic analysis of cDNA-expressed human CYP1A1, CYP1A2 and CYP1B1 with 7-ethoxyresorufin as substrate -- Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation -- Determination of the CYP2B6 component of 7-ethoxy-4-trifluoromethylcoumarin o-deethylation activity in human liver microsomes -- High-performance liquid chromatographic analysis of CYP2C8-catalyzed paclitaxel 6α-hydroxylation -- Determination of CYP2C9-catalyzed diclofenac 4'-hydroxylation by high-performance liquid chromatography -- CYP2C19-mediated (S)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromotography with radiometric detection -- CYP2D6-dependent bufuralol 1'-hydroxylation assayed by reversed-phase ion-pair high-performance liquid chromatography with fluorescence detection -- Spectrophotometric analysis of human CYP2E1-catalyzed p-nitrophenol hydroxylation -- Determination of CYP4A11-catalyzed lauric acid 12-hydroxylation by high-performance liquid chromatography with radiometric detection -- An isocratic high-performance liquid chromatographic assay for CYP7A1-catalyzed cholesterol 7α-hydroxylation -- Use of 7-ethoxycoumarin to monitor multiple enzymes in the human CYP1, CYP2 and CYP3 families -- Expression of eukaryotic cytochromes P450 in E. coli -- Expression of mammalian cytochromes P450 in yeast -- Expression of cytochromes P450 in a baculovirus system
Expression of cytochromes P450 in mammalisn cells -- Monoclonal antibodies to cytochromes P450 -- Targeting antipeptide antibodies towards cytochrome P450 enzymes -- Signals for retention of cytochrome P450 in the endoplasmic reticulum -- Quantification of cytochrome P450 gene expression by RNase protection analysis -- Hepatocyte cultures in drug metabolism and toxicological research and testing -- Isolation of rat hepatocytes -- Collagen-gel cultures of rat hepatocytes : collagen-gel sandwich and immobilization cultures -- Rat hepatocyte cultures : conventional monolayer cultures and cocultures with rat liver epithelial cells -- Isolation of periportal and pericentral hepatocytes -- Perifusion culture of hepatocytes -- Human hepatocyte culture -- Reconstruction of human epidermis in vitro -- Gene transfer and expression -- Lipid-mediated gene transfer into normal adult human hepatocytes in primary culture -- Identification of regulatory protein-binding sites in cytochrome P450 genes by means of the gel-retardation assay -- Cytochrome P450 gene regulation : analysis of protein-DNA interactions in situ -- Analysis of cytochrome P450 polymorphisms -- Induction of cytochrome P450 1A (CYP1A) in fish : a biomarker for environmental pollution -- Generation of allelic series in mice by gene targeting
edited by Ian R. Phillips and Elizabeth A. Shephard.
Includes bibliographical references and index.
Methods in molecular biology (Clifton, N.J.) ; v. 107