Lipoprotein protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1998]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 110. |
| Subjects: |
Table of Contents:
- PCR methodology applied to genetic studies of lipoprotein metabolism and atherosclerosis-- Separation of small-size DNA fragments using agarose gel electrophoresis
- Quantification of mRNA by polymerase chain reaction (PCR) using an internal standard and a nonradioactive detection method
- Generation of transgenic mice
- Preparation of knockout mice
- Fast ultracentrifugation methods for the separation of plasma lipoproteins
- Preparative isolation of plasma lipoproteins using fast protein liquid chromatography (FPLC)
- Separation of apolipoproteins by polyacrylamide gel electrophoresis
- Isolation and purification of serum lipoprotein (a) using lectin affinity
- Electrophoretic separation of LDL and HDL subclasses
- Measurement of lipoprotein particles
- Methodological approaches for assessing lipid and protein oxidation and modification in plasma and isolated lipoproteins
- Separation and quantitation of phospholipid classes by HPLC
- Assays of lecithin cholesterol acyltransferase (LCAT)
- Determination of the mass concentration and the activity of the plasma cholesteryl ester transfer protein (CETP)
- In vitro measurement of lipoprotein and hepatic lipases
- Cellular assays for lipoprotein receptors
- Measurements of proteoglycan-lipoprotein interaction by gel mobility shift assay