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Tissue in situ hybridization : methods in animal development /

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Bibliographic Details
Main Author: Jowett, Trevor
Corporate Author: European Molecular Biology Organization
Format: Book
Language:English
Published: New York : Heidelberg : Wiley ; Spektrum, [1997]
Subjects:
In Situ Hybridization > Laboratory Manuals.
Animals > embryology > Laboratory Manuals.
Table of Contents:
  • Principle of technique
  • Probe synthesis
  • Fixation
  • Hybridization to whole-mount embryos
  • Immunolocalization of probes
  • Alternative enzymes substrates
  • Two-color in situ hybridizations
  • Two-color in situ hybridization with fluorochromes
  • Embedding and sectioning whole-mount embryos
  • In situ hybridization on paraffin wax embedded tissue sections
  • In situ hybridization on cryostat sections of chick embryos
  • Whole-mount in situ hybridization of thick, floating tissue sections
  • Simultaneous localization of transcription and translation gene products
  • Special considerations, modifications, and problem troubleshooting
  • Photomicrography
  • Solutions, reagents, equipment, and suppliers