Protein synthesis : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1998]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 77. |
| Subjects: |
Table of Contents:
- Analyses of ribosome distribution during in vitro translation
- Allele-specific priming in the mapping of rRNA
- Permeablized mammalian cells as a system for protein synthesis
- The measurement of processivity errors in protein synthesis
- A biosynthetic approach for the incorporation of unnatural amino acids into proteins
- The analysis of translational activity using a reporter gene constructed from repeats of an antibody-binding domain from protein A
- In vitro engineering using acyl-derivatized tRNAs
- In vitro engineering using synthetic tRNAs with altered anticodons including four-nucleotide anticodons
- Analysis of rRNA function using specialized ribosomes
- Determination of the peptide elongation rate in vivo
- The measurement of missense errors during in vivo protein synthesis
- Heelprinting analysis of in vivo ribosome pause sites
- Analysis of elF-2α kinases in yeast
- Continuous-flow cell-free translation, transcription-translation and replication-translation systems
- Analysis of translation activity of extracts derived from oocytes and eggs of Xenopus laevis
- A fractionated reticulocyte lysate system for studies on protein synthesis initiation factors
- Measurement of ribosomal accuracy and proofreading in E. coli burst systems
- Measurement of rate of protein synthesis in vitro : preparation of Escherichia coli burst systems
- In vivo mutational analysis of ribosomal RNA in Saccharomyces cerevisiae
- Genetic selection of rRNA mutations
- Toeprinting assays : mapping by blocks to reverse transcriptase primer extension
- Mapping of pseudouridine residues in RNA to nucleotide resolution
- Chemical and enzymatic probing of antibiotic-ribosome complexes
- Protein engineering with nonstandard amino acids
- Internal ribosome entry sites tests with circular mRNAs
- Gel retardation and UV-crosslinking assays to detect specific RNA-protein interactions in the 5' or 3' UTRs of translationally regulated mRNAs
- Translational control of repressor proteins binding to the 5' UTR of mRNAs
- Identification and analysis of frameshift sites
- Synthesis and site-specific binding of thioated tRNAs to probe ribosome-tRNA interactions