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Tissue culture techniques : an introduction /

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Bibliographic Details
Main Author: Martin, Bernice M. (Bernice Michaelene), 1948-
Format: Book
Language:English
Published: Boston : Birkhäuser, [1994]
Subjects:
Tissue Culture.
Table of Contents:
  • Sterility
  • Aseptic technique
  • Sterilization methods
  • Quality control of sterilization
  • Routine cell culture
  • Feeding schedules and media components
  • Subcultivation
  • Cell enumeration and cell viability
  • Putting routine methods to work
  • Detecting and disposing of contamination
  • Troubleshooting
  • Safety
  • Experiments in culture
  • Alterations of the media
  • Substrata
  • Altering the environment
  • Primary cell culture
  • Isolation
  • Characterization
  • Cell preservation
  • Freezing the cells
  • Thawing the cells.
  • Cell cloning
  • Isolation
  • Assisting clonal survival
  • Culture changes
  • Loss of differentiated phenotype
  • Immortality and aging
  • Cell transformation
  • Transfection (gene transfer)
  • Information for new cell studies
  • Searching in the literature
  • Using advertising and catalogs
  • References, bibliographies and databases
  • Cell cycle
  • Media and salt solutions
  • Vendors.