Mycoplasma protocols /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1998]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 104. |
| Subjects: |
Table of Contents:
- Introduction
- The medical significance of mycoplasmas
- The veterinary significance of mycoplasmas
- Recovery of human mycoplasmas
- Recovery of mycoplasmas from animals
- Recovery of mycoplasmas from birds
- Cultivation of ureaplasmas
- Quality control testing of mycoplasma media
- Biochemical characteristics in mycoplasma identification
- Enzyme analysis : the rationale and use of enzyme assays in assigning function to gene nucleotide sequences and the procedures for the assay of three enzymatic functions conserved in mollicutes
- Determination of substrate utilization rates by mycoplasmas
- Serological identification of mycoplasmas by growth and metalbolism inhibition tests
- Identification of mycoplasmas by dot immunobinding on membrane filtration (MF Dot)
- Identification of mycoplasmas by immunoflurescence
- Diagnostic application of monoclonal antibody (MAb)-based sandwich ELISAs
- Immunohistochemical staining of fixed tissues
- Extraction of DNA from mycoplasmas
- Characterization of mycoplasmas by PCR and sequence analysis with universal 16S rDNA primers
- PCR and RFLP methods for specific detection and identification of mycoplasma mycoides subsp. mycoides SC
- Characterization of mycoplasmas by RAPD fingerprinting
- Classification of isolates by DNA-DNA hybridization
- Insertion sequence analysis
- Detection of mycoplasmas in cell cultures by cultural methods
- Detection of mycoplasmas in cell culture by fluorescence methods
- Transformation of mycoplasmas
- Transposon mutagenesis
- Demonstration of extrachromosomal elements
- Expression of foreign genes in acholeplasma laidlawii
- Mycoplasma gene expression in Escherichia coli
- Polyacrylamide gel-electrophoresis seperation of whole-cell proteins
- Immunological and biochemical characterization of membrane proteins
- Detection and analysis of mycoplasma adhesins
- Transmission electron microscopy and immunogold staining of mollicute surface antigens