Pichia protocols /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1998]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 103. |
| Subjects: |
Table of Contents:
- Introduction to Pichia pastoris
- Classical genetic manipulation
- Transformation
- Small vectors for expression based on dominant drug resistance with direct multicopy selection
- The generation of multicopy recombinant strains
- Isolation of nucleic acids
- Generation of pretease-deficient strains and their use in hererologous protein expression
- Glycosylation profiling of heterologous proteins
- High cell-density fermentation
- Use of Pichia pastoris as a model eukaryotic system : peroxisome biogenesis
- Secretion of recombinant human insulin-like growth factor I (IGF-I)
- Secretion of scFv antibody fragments
- Expression of tetanus toxin fragment C
- Expression of EGF and HIV envelope glycoprotein
- Expression of an integral membrane protein, the 5HT̐₅ receptor
- Localization of the myc-tagged 5HT̐₅ receptor by immunogold staining of ultrathin sections