Animal cell culture techniques /

Animal information

Bibliographic Details
Other Authors: Clynes, M. (Martin)
Format: Book
Language:English
Published: Berlin ; New York : Springer, [1998]
Series:Springer lab manual.
Subjects:
Table of Contents:
  • Pt. 1. General methods
  • Cloning animal cells / Shirley McBride, Mary Heenan, Martin Clynes
  • Cell culture and diagnostic virology / Edwin O' Kelly
  • Screening for mycoplasma contamination in animal cell cultures / Mary Heenan and Martin Clynes
  • Serum-free media / Joanne Keenan, Paula Meleady, and Martin Clynes
  • Screening culture media and sera for endotoxin testing / Joanne Keenan and Martin Clynes
  • Generation of monoclonal antibodies and characterization of novel antibodies by western blotting and immunocytochemistry / Elizabeth Moran .. [et. al.].
  • The Generation of long-term human antibody-producing cell lines using the epstein-barr virus / Olivia Flynn and Dermot Walls
  • Primary culture / Finbar O' Sullivan ... [et. al.]
  • Pt. 2. Cell proliferation and death
  • Baculoviral expression and partial purification of cyclin/cdk complexes / Mark Jackman
  • Methods for studying phosphoinositide metabolism in cultured cells / Conor Duffy, Ailish Hynes, and Leo Quinlan
  • Analysis of cell cycle and cell death mechanisms / S. Verhaegen ... [et. al.]
  • Pt. 3. In Vitro models for cell differentiation.
  • Assessing release of secretory products from individual cells / Domenico Bosco and Paolo Meda
  • The Granulocyte/macrophage colony-forming unit assay / Augusto Pessina
  • Investigations into cell differentiation using cells in culture / Shirley McBride and Martin Clynes
  • Cell culture models for mononuclear phagocytes / Philippe Poindron and Yves Lombard
  • The Human leukemia cell line hl-60 as a cell culture model to study neutrophil functions and inflammatory cell responses / Faustino Mollinedo, Antonio M. Santos-Beneit, and Consuelo Gajate
  • Cell culture models for polarized epithelial monolayers / Alan W. Baird and Ena S. Prosser
  • Ion channels and cell signaling in cell cultures / Valerie Urbach ... [et. al.]
  • Isolation, cultivation and differentiation of lung type II epithelial cells / Paula Meleady ... [et. al.]
  • Culture systems for hepatocytes for use in toxicology and differentiation studies / Gennady Ilyin ... [et. al.]
  • In Vitro models for toxicology and pharmacology
  • Cellular models for in vitro toxicity testing / Flavia Zucco, Isabella de Angelis, and Annalaura Stammati
  • Miniaturized in vitro methods in toxicity testing / Robert O' Connor, ... [et. al.]
  • Investigation of anti-tumor drug accumulation and efflux in cell culture / Irene Cleary and Martin Clynes
  • Current status of genetically engineered in vitro systems for biotransformation / Johannes Doehmer.
  • Pt. 5. Industrial application of animal cell culture
  • Scale-up of animal cell cultures / Eunan McGlinchey, Donnacha O' Driscoll and Martin Clynes
  • Purification of monoclonal antibodies produced by animal cells in culture / Conor O' Dea, Suzanne O' Connor, and Martin Clynes
  • Biosafety assessment of cultured animal cells used for biopharmaceutical producation / Carl N. Martin and David W. Birch
  • Cytokine cell culture assays / Alice Redmond, Jacqueline Quinn, and Susan Molloy
  • Pt. 6. Genetic manipulation and analysis of human and animal cells in culture.
  • Antisense methods in cell culture / Daragh Byrne, Carmel Daly, and Martin Clynes
  • Transfection with ribozymes to investigate the role of specific gene expression / Carmel Daly, Daragh Byrne, and Martin Clynes
  • Methods for studying the transcription and dnase I hypersensitive sites of genes in cells in culture / Noel Daly and Martin Clynes
  • Analysis of specific and differentially expressed mrnas in cell culture / Toni O' Doherty, Roisin Nic Amhlaoibh, and Martin Clynes
  • Human gene therapy: review and outlook / Gerard J. McGarrity.