Gene targeting protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2000]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 133. |
| Subjects: |
Table of Contents:
- Nucleic acid transfer using cationic lipids
- Optimizing polyethylenimine-based gene transfer into mammalian brain for analysis of promoter regulation and protein function
- Gene transfer and drug delivery by electronic pulse delivery: a nonviral delivery approach
- Strategies for improving the frequency and assessment of homologous recombination
- Effective gene transfer using viral vectors based on SV40
- Rapid generation of isogenic mammalian cell lines expressing recombinant transgenes by use of Cre recombinase
- Site-directed alteration of genomic DNA by small-fragment homologous replacement
- Mutation correction by homologous recombination with an adenovirus vector
- Site-specific targeting of DNA plasmids to chromosome 19 using AAV Cis and Trans sequences
- Adeno-associated virus based gene therapy in skeletal muscle
- Rapid establisment of myeloma cell lines expressing Fac(Tac)-protamine, a targetable protein vector, directed against high-affinity α-chain of human interleukin-2 receptor
- EBV-derived episomes to probe chromatin structure and gene expression in human cells
- Triplex-directed site-specific genome modification
- Use of quantitative ligation-mediated polymerase chain reaction to detect gene targeting by alkylating oligodeoxynucleotides
- Gene targeting in plants via site-directed mutagenesis
- Antisense oligonucleotides as modulators of pre-mRNA splicing.