http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
x, 353 pages : illustrations (some color) ; 24 cm.
Introduction to the polymerase chain reaction -- Setting up a PCR laboratory -- Amplification from archival materials -- Quantitative PCR -- Mutation screening using PCR-SSCP : silver staining and isotopic protocols -- Multiple fluorescence-based PCR-SSCP analysis with primer-, post- and internal labelling -- The amplification refractory mutation system -- Artificial restriction fragment length polymorphism (A-RFLP) analysis -- Long range PCR -- Generation of labeled probes by PCR -- PCR for the detection of minority DNA populations -- In situ amplification -- Sequencing of PCR products -- Reverse transcriptase-polymerase chain reaction (RT-PCR) -- PCR amplification of minisatellite DNA for the detection of mixed chimerism after bone marrow transplantation -- Diagnosis of mitochondrial disorders using the PCR -- PCR analysis of CD44 variants in tumors -- Detection of circulating solid tumor cells by reverse transciptase polymerase chain reaction -- Detection of circulating tumor cells using immunobead-PCR -- Single cell PCR : theory, practice and clinical applications -- PCR-based noninvasive prenatal diagnosis using fetal cells in maternal circulation -- Noninvasive prenatal diagnosis using a single fetal nucleated erythrocyte isolated by micromanipulation for maternal blood -- Diagnosis of chromosomal aneuploidies using quantitative fluorescent PCR -- Detection of the hepatitis c virus by RT-PCR -- The application of PCR to the detection of M. tuberculosis in sputum samples -- Microsatellite analysis in human disease
Includes bibliographical references and index.