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Ultracytochemistry of intracellular membrane glycoconjugates /

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Bibliographic Details
Main Author: Neiss, Wolfram F. (Wolfram Friedrich), 1953-
Format: Book
Language:English
Published: Berlin ; New York : Springer-Verlag, [1986]
Series:Advances in anatomy, embryology, and cell biology ; v. 99.
Subjects:
Cytoplasmic Granules > ultrastructure.
Intracellular Membranes > ultrastructure.
Carbohydrates > analysis.
Table of Contents:
  • Animals and animal experiments
  • Fixation
  • Postfixation, osmium-low ferrocyanide reaction
  • Dehydration and embedding
  • Cytochemistry of carbohydrates in ultrathin sections
  • PTA-HCL reaction
  • PA-TCH-Ag reaction
  • mPA-TCH-Ag reaction
  • HID-TCH-Ag reaction
  • Electron microscopy
  • Results
  • Lysosomes
  • Heterolysosomes on untreated animals
  • Heterolysosomes induced by triton WR-1339
  • Autophagic vacuoles
  • Multivesicular bodies
  • Eosinophilic leukocytes
  • Zymogen granules of exocrine glands
  • Submandibular gland
  • Parotid gland
  • Pancreas
  • Paneth cells
  • Secretory granules of endocrine glands
  • Adenohypophysis
  • Neurohypophysis
  • Gastroenteropancreatic endocrine cells
  • Adrenal medulla
  • Mast cell granules
  • Mitochondria and peroxisomes
  • Specificity controls
  • Critique of the methods
  • Pretreatment of the tissue
  • Cytochemical methods for the demonstration of carbohydrates
  • Evaluation of the cytochemical results
  • Inner membrane coat of lysosomes and secretory granules
  • Matrix of lysosomes and secretory granules
  • Mitochondria, peroxisomes, and golgi apparatus