Ex vivo cell therapy /

Bibliographic Details
Other Authors: Schindhelm, Klaus, Nordon, Robert
Format: Book
Language:English
Published: San Diego : Academic Press, [1999]
Subjects:
Table of Contents:
  • Introduction / Robert E. Nordon, Klaus Schindhelm
  • Transcriptional control of hematopoiesis / L. Robb, A.G. Elefanty, C.G. Begley
  • SCL: a master hematopoietic regulator gene identified via chromosome translocation
  • SCL in normal hematopoiesis
  • LMO2: another partner for SCL
  • The GATA family of transcriptional factors
  • AML-1 and CBFβ are required for definitive hematopoiesis
  • Additional examples of defective hematopoiesis: MYB and PU.1 knockouts
  • Lineage-specific defects: EKLF and NF-E2 knockouts
  • Ikaros and E2A: examples of key transcription factors in lymphopoiesis
  • Conclusions
  • Cell signaling by hematopoietic growth factor receptors / R. Starr, N.A. Nicola
  • Introduction
  • The JAK/STAT signal transduction pathways
  • Ras activation pathway
  • Signal transduction through phospholipase C
  • Inhibitory pathways
  • Pathways leading to cell death (apoptosis) or survival
  • TGF-β family signaling
  • Some other pathways
  • Summary
  • Influence of cytokines and adhesion molecules on hematopoietic stem cell development / P.J. Simmons, D.N. Haylock, J.-P. Lévesque
  • Introduction
  • Primitive hematopoietic progenitor cells require multiple cytokines for proliferation
  • Cytokines which act on primitive HPC: Flt3-ligand and thrombopoietin
  • What then for ex vivo manipulation of hematopoietic stem cells?
  • Adhesive interactions and their role of hematopoietic regulation
  • The cross-talk between integrins and cytokine receptors
  • Integrins and hematopoietic leukemias: the example of chronic myelogenous leukemia (CML)
  • Mucin-like molecules: an emerging family of cell adhesion molecules expressed by hematopoietic progenitor cells
  • Outside-in signaling through mucin-like molecules on hematopoietic progenitor cells: negative regulators of hematopoiesis?
  • Allogeneic hematopoietic stem cell transplantation / P.A. Rowlings
  • Introduction
  • Indications for allogeneic hematopoietic stem cell transplants
  • Donor and tissue sources of hematopoietic stem cells
  • Results of allogeneic hematopoietic stem cell transplants
  • Allogeneic donor lymphocyte infusions
  • Adverse events in long-term survivors
  • Autologous stem cell transplantation / Uwe Hahn, L. Bik To
  • Introduction and historical background
  • Hematological malignancies
  • Solid tumors
  • Peripheral blood stem cell transplantation
  • Malignant contamination of autologous grafts
  • Future developments
  • Cord blood stem cell transplantation / Marcus R. Vowels
  • Introduction
  • Unrelated bone marrow donor transplants
  • Cord blood
  • Related cord blood transplants
  • Unrelated cord blood transplants
  • Unrelated cord blood banking
  • Advantages and disadvantages of cord blood
  • Conclusions and future progress
  • Reconstitution of immunity by adoptive immunotherapy with T cells / Stanley R. Riddell ... [et al.]
  • Introduction
  • Biological and molecular basis of T-cell recognition of viruses and tumors
  • Immunologic memory mediated by αβ T cells
  • Mechanisms of evasion of T-cell responses by viruses and tumors
  • Methods of selection of antigen-specific T-cell clones
  • Genetic modification of T cells to improve the safety and/or efficacy of adoptive immunotherapy
  • Clinical studies of cellular adoptive immunotherapy for viruses and virus-induced malignancies
  • Potential for adoptive immunotherapy with tumor-reactive T cells
  • Exogenous gene transfer into lymphoid and hematopoietic progenitor cells / Lun Quan Sun, Michelle Miller, Geoff Symonds
  • Introduction
  • Vector design
  • Packaging cell lines
  • Gene transfer into lymphoid cells
  • Gene transfer into hematopoietic progenitor cells
  • Process development for ex vivo cell therapy / Charles Dowding ... [et al.]
  • Introduction
  • Clinical utility of transplantation of purified HSCs
  • Regulation of ex vivo cell processing
  • Quality assurance
  • Process development
  • Cell separation / Robert E. Nordon, Klaus Schindhelm
  • Introduction
  • Cell separation methods
  • Rationale for cell selection
  • Environmental requirements of hematopoietic progenitor cells in ex vivo expansion systems / Peter W. Zandstra, Connie J. Eaves, James M. Piret
  • Introduction
  • Potential applications of cell expansion as a cellular therapy
  • Development of hematopoietic cell culture systems suitable for clinical scale-up
  • Refinement of bioreactor operating parameters
  • Receptor stimulation and medium cytokine depletion
  • Ex vivo hematopoietic cell expansion for bone marrow transplantation / Ramkumar Mandalam, Manfred Koller, Alan Smith
  • Introduction
  • Hematopoietic stem cell transplantation
  • Definition of a biological process for HSC production
  • Design of a device to implement the biological process
  • Integration of device into a novel GMP cell production system
  • Biological performance of the automated AastromReplicell system
  • Implementation of the process and AastromReplicell system in clinical trials
  • Gene delivery technology: nonviral and viral vector systems / Mitch Raponi, Geoff Symonds
  • Introduction
  • Nonviral gene delivery
  • Viral delivery systems
  • Summary and future directions / Robert E. Nordon, Klaus Schindhelm
  • Introduction
  • Extrinsic or intrinsic manipulation of hematopoietic cell growth and differentiation
  • Hematopoietic cell graft engineering
  • Core technologies required for delivery of ex vivo cell therapy
  • Future directions.