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A guide to protein isolation /

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Bibliographic Details
Main Author: Dennison, Clive
Format: Book
Language:English
Published: Dordrecht ; Boston : Kluwer Academic, [1999]
Subjects:
Proteins > isolation & purification.
Table of Contents:
  • An overview of protein isolation
  • Why do it?
  • Properties of proteins
  • The conceptual basis of protein isolation
  • The purification table
  • Assay extraction and sub-cellular fractionation
  • Buffers
  • Assays for activity
  • Assay for protein content
  • Methods for extraction of proteins
  • Clarification of the extract
  • Centrifugal sub-cellular fractionation
  • Concentration of the extract
  • Freeze drying
  • Dialysis
  • Ultrafiltration
  • Concentration/fractionation by salting out
  • Fractional precipitation with polyethylene glycol
  • Precipitation with organic solvents
  • Dye precipitation
  • Chromatography
  • Principles of chromatography
  • Equipment for low pressure liquid chromatography
  • Ion-exchange chromatography (IEC)
  • Chromatofocusing
  • Molecular exclusion chromatography (MEC)
  • Hydroxyapatite chromatography
  • Affinity chromatography
  • Hydrophobic interaction (HI) chromatography
  • Principles of electrophoresis
  • Boundary (tiselius) electrophoresis
  • Paper electrophoresis
  • Cellulose acetate membrane electrophoresis
  • Agarose gel electrophoresis
  • Starch gel electrophoresis
  • Polyacrylamide gel electrophoresis (PAGE)
  • SDS-PAGE
  • Pore gradient gel electrophoresis
  • Isoelectric focusing
  • 2-D electrophoresis
  • Non-linear electrophoresis
  • Immunological methods
  • The structure of antibodies
  • Antibody production
  • Immunoprecipitation
  • Immunoelectrophoresis
  • Amplification methods.