Diagnostic and therapeutic antibodies /

Bibliographic Details
Other Authors: George, Andrew J. T., Urch, Catherine E.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2000]
Series:Methods in molecular medicine ; 40.
Subjects:
Table of Contents:
  • Pt. 1. Introduction
  • The antibody molecule / Andrew J.T. George
  • Polyclonal and monoclonal antibodies / Mary A. Ritter
  • Engineering antibody molecules / Rakesh Verma, Ekaterini Boleti
  • Phage display technology / Michael Johns
  • Pt. 2. Antibodies in medicine
  • Prospects for the application of antibodies in medicine / Herman Waldmann
  • Antibodies for neoplastic disease: solid tumours / Ian T.W. Matthews
  • The application of monoclonal antibodies in the treatment of lymphoma / Martin J. Glennie ... [et al.]
  • Antibodies for inflammatory disease: effector cells / Richard Smith
  • Antibodies for inflammatory disease: cytokines / Peter C. Taylor
  • Antibodies for transplantation / Denise L. Faustman
  • Antibody-based therapies in infectious diseases / H. Barbaros Oral, Cezmi A. Akdis̜
  • Antibodies in nuclear medicine / A. Michael Peters
  • Animal models for tumor localization / Gail Rowlinson-Busza
  • Antibodies for immunoassays / David J. Newman
  • Pt. 3. Ethics and industry
  • Intellectual property / William M. Brown
  • From laboratory to clinic: the story of CAMPATH-1 / Geoff Hale, Herman Waldmann
  • Pt. 4. Production and purification
  • Production of monoclonal antibodies / Jóna Freysdóttir
  • Purification of monoclonal antibodies using protein A/G / Bridget Heelan
  • Preparation of monoclonal antibodies using ion exchange chromatography / Maureen Power
  • Quality control of raw materials / Patrick Harrison, Geoff Hale
  • Cell banks and stability of antibody production / Pru Bird, Geoff Hale
  • Measurement of endotoxin / Jenny Phillips, Patrick Harrison, Geoff Hale
  • Aseptic vial filling / Kuldip Bhamra ... [et al.]
  • Measurement of antibody concentrations by hemagglutination / Jenny Phillips, Geoff Hale
  • Pt. 5. Clinical application of antibodies
  • Enzymatic digestion of monoclonal antibodies / Sarah M. Andrew
  • How to make bispecific antibodies / Ruth R. French
  • Radiolabeling of monoclonal antibodies / Calvin S.R. Gooden
  • Determination of the immunoreactivity of radiolabeled monoclonal antibodies / Gail Rowlinson-Busza
  • Use of biosensors to measure the kinetics of antibody-antigen interactions / Andrew J.T. George
  • Pt. 6. Application of antibodies in vitro
  • How to set up an ELISA / Bill Jordan
  • Measurement of HAMA and anti-idiotypic antibodies / Steve Nicholson
  • SDS-PAGE and western blotting / Abdulhamid A. Al-Tubuly
  • Flow cytometric analysis / Paul F. McKay
  • Immunocytochemistry / Susan Van Noorden
  • Immunolabeling for electron microscopy / Catherine E. Sarraf
  • Pt. 7. Antibody engineering
  • PCR of the V region / Rakesh Verma
  • Phage display technology: protocols / Michael Johns, Donald B. Palmer.