Polyamine protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1998]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 79. |
| Subjects: |
Table of Contents:
- Pt. 1. Polyamines: an introduction / David M.L. Morgan
- Pt. 2. Assay methods for enzymes of polyamine biosynthesis
- Determination of ornithine decarboxylase activity using H3 ornithine / Amalia Tabib
- Assay of mammalian ornithine decarboxylase activity using C14 ornithine / Catherine S. Coleman and Anthony E. Pegg
- Assay of mammalian S-adenosylmethionine decarboxylase activity / Lisa M. Shantz and Anthony E. Pegg
- Assay of spermidine and spermine synthases / Laurie Wiest and Anthony E. Pegg.
- Measurement of spermidine,spermine N1-cetyltransferase activity / Heather M. Wallace and Deborah M. Evans
- Assay of spermidine N- acetyltransferase / Jim Blankenship
- Pt. 3. Assay methods for enzymes of polyamine catabolism
- Assay of N8-acetylspermidine deacetylase / Jim Blankenship
- Hydrogen peroxide assay for amine oxidase activity / R. James Storer and Antonio Ferrante
- Radiochemical assay of diamine oxidase / R. James Storer and Antonio Fettante.
- Radiochemical estimation of polyamine oxidase / David M.L. Morgan
- Measurement of aldehyde formation as an assay for polyamine oxidase activity / David M.L. Morgan
- Pt. 4. Measurement of polyamines
- Determination of polyamines as their benzoylated derivatives by HPLC / David M.L. Morgan
- A Dansyl chloride- HPLC method for the determination of polyamines / Karl J. Hunter.
- The Determination of polyamines and amino acids by a fluorescamine-HPLC method / Karl J. Hunter and Alan H. Fairlamb
- Thin-layer chromatographic method for assaying polyamines / Rentala Madhubala
- Pt. 5. Measurement of polyamine transport
- Measurement of polyamine transport: adherent cells / David M.L. Morgan
- Measurement of polyamine transport: cells in suspension / Sarah A. Le Quesne and Alan H. Fairlamb
- Measurement of polyamine efflux from cells in culture / Heather M. Wallace and A. Jill Mackarel.
- Pt. 6. Measurement of polyamine effects on cell growth
- Leucine incorporation and thymidine incorporation / Christopher P. Denton
- Tetrazolium MTT assay for cellular viability and activity / David M.L. Morgan.