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Basic methods in molecular biology /

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Bibliographic Details
Main Author: Davis, Leonard G.
Other Authors: Kuehl, W. Michael, Battey, James F.
Format: Book
Language:English
Published: Norwalk, Conn. : Appleton & Lange, [1994]
Edition:Second edition.
Subjects:
Molecular Biology > methods.
Table of Contents:
  • General methods
  • Bacterial strains and cloning vectors
  • Enzymes that modify DNA and RNA
  • In vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase
  • DNA restriction fragment analysis and preparation
  • In vitro labeling of probes and filter hybridization
  • Plasmid DNA preparation from E coli hosts
  • Preparation of DNA from lambda bacteriophage clones
  • Subcloning fragments into plasmid vectors and plasmid construction
  • Preparation of genomic DNA
  • Preparation and analysis of RNA from eukaryotic cells
  • Genomic cloning
  • Preparation and analysis of cDNA libraries
  • Preparation of subtractive cDNA
  • Chain termination sequencing
  • Transfection of mammalian cells
  • Basic methods of protein analysis
  • In situ hybridization
  • Transgenic mouse preparation
  • Detection and in vitro generation of specific mutations in genes and cDNAs
  • Preparation of stock solutions
  • Generally useful information
  • Suppliers of reagents and equipment.