Cytotoxic drug resistance mechanisms /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1999]
|
| Series: | Methods in molecular medicine ;
28. |
| Subjects: |
Table of Contents:
- Drug resistance: the clinical perspective
- Cell sensitivity assays: clonogenic assay
- Cell sensitivity assays: the MTT assay
- Cell sensitivity assays: detection of apoptotic cells in vitro using the TUNEL assay
- Analysis of apoptosis in tissue sections
- Measurement of P-glycoprotein function
- Measuring MDR-1 by quantitative RT-PCR
- Microtiter plate technique for the measurement of glutathione in fresh and cryopreserved lymphoblasts using the enzyme recycling method
- Measurement of reduced glutathione using high-pressure liquid chromatography
- Topoisomerase I and II activity assays
- 5-fluorouracil metabolising enzymes
- Measuring DNA adducts by immunoassay (ELISA)
- Measuring drug-DNA adducts in individual cells
- Measurement of drug-induced DNA interstrand crosslinking using the single-cell gel electrophoresis (Comet) assay
- PCR analysis of microsatellite instability
- O⁶-alkylguanine-DNA alkyltransferase assay
- Analysis of the p53 status of tumors: an overview of methods
- Bcl-2 family immunohistochemistry
- Genetic analysis of drug resistance by fluorescence in situ hybridization
- Genetic analysis of drug resistance by reverse in situ hybridization.