rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Basic molecular biology
    sub title: essential techniques
  name:
    name part: Darbre, Philippa D.
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: enk
    date issued: 1999
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xi, 193 pages ; 22 cm.
  table of contents: DNA structure -- Denaturation and renaturation (hybridization) of -- DNA -- RNA structure -- Enzymes used in molecular biology -- Restriction endonucleases -- DNA polymerases -- RNA polymerases -- Nucleases -- End-modification enzymes -- Ligases -- DNA cloning -- Setting up a molecular biology laboratory -- Preparation of DNA -- Methods available -- Protocols -- Preparation of cell pellet and cell lysis -- Phenol extraction of DNA -- Ethanol precipitation of DNA -- Removal of RNA by ribonuclease treatment -- Final purification steps -- Assessment of DNA concentration and DNA quality -- Preparation of RNA -- Methods available -- Protocols -- Preparation of cell pellet -- Preparation of cytoplasmic RNA -- Preparation of whole cell RNA by ultracentrifugation method -- Preparation of whole cell RNA by Chomczynski-Sacchi method -- Assessment of RNA concentration and RNA quality -- Selection of poly(A)* RNA -- Preparation of plasmid DNA -- Methods available -- Protocols -- Transformation of bacteria with plasmid -- Growth of bacteria and amplification of plasmid -- Small-scale preparation of plasmid DNA -- Large-scale preparation of plasmid DNA -- Isolation of cloned gene sequences -- DNA analysis by restriction enzyme digestion and Southern blotting -- Methods available -- Protocols -- Restriction endonuclease digestion -- Concentration of DNA by ethanol precipitation -- Agarose gel electrophoresis -- Southern blotting -- Radiolabeling of DNA probe -- Hybridization of blot -- Washing of blot -- 5'-end radiolabeling of DNA molecular weight markers -- DNA analysis by PCR -- Methods available -- Protocols -- PCR reaction -- Analysis of PCR products by agarose gel electrophoresis -- RNA analysis by Northern blotting -- Methods available -- Protocols -- Formaldehyde-agarose gel electrophoresis -- Northern blotting and hybridization to radiolabelled DNA probe -- RNA analysis by RNase protection -- Methods available -- Protocols -- Preparation of riboprobe -- RNase protection a…
  note: Philippa D. Darbre.
  note: Includes bibliographical references and index.
  subject:
    topic: Molecular Biology
    genre: Laboratory Manuals
  classification: QH506 .D355 1999
  classification: 572.8
  related item:
    title info:
      title: Essential techniques series
  identifier: 0471977055 (alk. paper)
  identifier: 98027199
  record info:
    record content source: DLC
    record creation date: 980618
    record change date: 20191203081753.0
    record identifier: in00004485697