http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
DNA structure -- Denaturation and renaturation (hybridization) of -- DNA -- RNA structure -- Enzymes used in molecular biology -- Restriction endonucleases -- DNA polymerases -- RNA polymerases -- Nucleases -- End-modification enzymes -- Ligases -- DNA cloning -- Setting up a molecular biology laboratory -- Preparation of DNA -- Methods available -- Protocols -- Preparation of cell pellet and cell lysis -- Phenol extraction of DNA -- Ethanol precipitation of DNA -- Removal of RNA by ribonuclease treatment -- Final purification steps -- Assessment of DNA concentration and DNA quality -- Preparation of RNA -- Methods available -- Protocols -- Preparation of cell pellet -- Preparation of cytoplasmic RNA -- Preparation of whole cell RNA by ultracentrifugation method -- Preparation of whole cell RNA by Chomczynski-Sacchi method -- Assessment of RNA concentration and RNA quality -- Selection of poly(A)* RNA -- Preparation of plasmid DNA -- Methods available -- Protocols -- Transformation of bacteria with plasmid -- Growth of bacteria and amplification of plasmid -- Small-scale preparation of plasmid DNA -- Large-scale preparation of plasmid DNA -- Isolation of cloned gene sequences -- DNA analysis by restriction enzyme digestion and Southern blotting -- Methods available -- Protocols -- Restriction endonuclease digestion -- Concentration of DNA by ethanol precipitation -- Agarose gel electrophoresis -- Southern blotting -- Radiolabeling of DNA probe -- Hybridization of blot -- Washing of blot -- 5'-end radiolabeling of DNA molecular weight markers -- DNA analysis by PCR -- Methods available -- Protocols -- PCR reaction -- Analysis of PCR products by agarose gel electrophoresis -- RNA analysis by Northern blotting -- Methods available -- Protocols -- Formaldehyde-agarose gel electrophoresis -- Northern blotting and hybridization to radiolabelled DNA probe -- RNA analysis by RNase protection -- Methods available -- Protocols -- Preparation of riboprobe -- RNase protection assay -- Analysis of products by polyacrylamide gel electrophoresis -- RNA analysis by RT-PCR and differential display -- Methods available -- Protocols -- RT-PCR reaction and analysis of products -- Differential display -- Analysis of protein-DNA interactions by gel shift assay -- Methods available -- Protocols -- Preparation of protein extract -- Gel shift assay -- Gene transfection -- Methods used -- Protocols -- Transient transfection -- Stable transfection -- Appendix A: recipes for routine solutions -- Appendix B: Sources of reagents and equipment.
Includes bibliographical references and index.