| Tag |
First Indicator |
Second Indicator |
Subfields |
| LEADER |
00000cam a2200000 a 4500 |
| 001 |
in00004485697 |
| 005 |
20191203081753.0 |
| 008 |
980618s1999 enk b 001 0 eng |
| 010 |
|
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|a 98027199
|
| 015 |
|
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|a GB99-841
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| 020 |
|
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|a 0471977055 (alk. paper)
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| 035 |
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|a (OCoLC)39335670
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| 035 |
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|9 AAH7811MS
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| 035 |
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|z (UtOrBLW)26761
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| 035 |
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|a 40728
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| 040 |
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|a DLC
|c DLC
|d UKM
|d C#P
|d AGL
|d COU
|d UtOrBLW
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| 049 |
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|a TMVM
|c 1
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| 050 |
0 |
0 |
|a QH506
|b .D355 1999
|
| 072 |
|
0 |
|a X300
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| 082 |
0 |
0 |
|a 572.8
|2 21
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| 096 |
|
|
|a QH 506
|b D213b 1999
|
| 100 |
1 |
|
|a Darbre, Philippa D.
|
| 245 |
1 |
0 |
|a Basic molecular biology :
|b essential techniques /
|c Philippa D. Darbre.
|
| 264 |
|
1 |
|a Chichester ;
|a New York :
|b Wiley,
|c [1999]
|
| 264 |
|
4 |
|c ©1999
|
| 300 |
|
|
|a xi, 193 pages ;
|c 22 cm.
|
| 336 |
|
|
|a text
|2 rdacontent
|b txt
|
| 337 |
|
|
|a unmediated
|2 rdamedia
|
| 338 |
|
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|a volume
|2 rdacarrier
|b nc
|
| 490 |
1 |
|
|a Essential techniques series
|
| 504 |
|
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|a Includes bibliographical references and index.
|
| 505 |
0 |
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|a DNA structure -- Denaturation and renaturation (hybridization) of -- DNA -- RNA structure -- Enzymes used in molecular biology -- Restriction endonucleases -- DNA polymerases -- RNA polymerases -- Nucleases -- End-modification enzymes -- Ligases -- DNA cloning -- Setting up a molecular biology laboratory -- Preparation of DNA -- Methods available -- Protocols -- Preparation of cell pellet and cell lysis -- Phenol extraction of DNA -- Ethanol precipitation of DNA -- Removal of RNA by ribonuclease treatment -- Final purification steps -- Assessment of DNA concentration and DNA quality -- Preparation of RNA -- Methods available -- Protocols -- Preparation of cell pellet -- Preparation of cytoplasmic RNA -- Preparation of whole cell RNA by ultracentrifugation method -- Preparation of whole cell RNA by Chomczynski-Sacchi method -- Assessment of RNA concentration and RNA quality -- Selection of poly(A)* RNA -- Preparation of plasmid DNA -- Methods available -- Protocols -- Transformation of bacteria with plasmid -- Growth of bacteria and amplification of plasmid -- Small-scale preparation of plasmid DNA -- Large-scale preparation of plasmid DNA -- Isolation of cloned gene sequences -- DNA analysis by restriction enzyme digestion and Southern blotting -- Methods available -- Protocols -- Restriction endonuclease digestion -- Concentration of DNA by ethanol precipitation -- Agarose gel electrophoresis -- Southern blotting -- Radiolabeling of DNA probe -- Hybridization of blot -- Washing of blot -- 5'-end radiolabeling of DNA molecular weight markers -- DNA analysis by PCR -- Methods available -- Protocols -- PCR reaction -- Analysis of PCR products by agarose gel electrophoresis -- RNA analysis by Northern blotting -- Methods available -- Protocols -- Formaldehyde-agarose gel electrophoresis -- Northern blotting and hybridization to radiolabelled DNA probe -- RNA analysis by RNase protection -- Methods available -- Protocols -- Preparation of riboprobe -- RNase protection assay -- Analysis of products by polyacrylamide gel electrophoresis -- RNA analysis by RT-PCR and differential display -- Methods available -- Protocols -- RT-PCR reaction and analysis of products -- Differential display -- Analysis of protein-DNA interactions by gel shift assay -- Methods available -- Protocols -- Preparation of protein extract -- Gel shift assay -- Gene transfection -- Methods used -- Protocols -- Transient transfection -- Stable transfection -- Appendix A: recipes for routine solutions -- Appendix B: Sources of reagents and equipment.
|
| 650 |
|
2 |
|a Molecular Biology
|v Laboratory Manuals.
|
| 830 |
|
0 |
|a Essential techniques series.
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| 999 |
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|a MARS
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| 999 |
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| 952 |
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|p ric
|a Texas A&M University
|b Rellis Campus
|c Joint Library Facility
|d Remote Storage
|t 0
|e QH 506 D213b 1999
|h Other scheme
|i unmediated -- volume
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