In vitro mutagenesis protocols /

Bibliographic Details
Other Authors:	Trower, Michael K.
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [1996]
Series:	Methods in molecular biology (Clifton, N.J.) ; v. 57.
Subjects:	Mutagenesis, Insertional > methods. Mutagenesis, Site-Directed. Mutagenesis. Polymerase Chain Reaction > methods.

Table of Contents:

Site-directed mutagenesis using positive antibiotic selection
In vitro site-directed mutagenesis using the unique restriction site elimination (USE) method
Site-directed mutagenesis using double-stranded plasmid DNA templates
Site-directed mutagenesis using a uracil-containing phagemid template
Oligonucleotide-directed mutagenesis using an improved phosphorothioate approach
Analysis of point mutations by use of amber stop codon suppression
A simple method for site-directed mutagenesis with double-stranded plasmid DNA
Double-stranded DNA site-directed mutagenesis
Solid-phase in vitro mutagenesis using a plasmid DNA template
Targeted mutagenesis mediated by the triple helix formation
A universal nested deletion method using an arbitrary primer and elimination of a unique restriction site
Ordered deletions using exonuclease III
Ligase chain reaction for site-directed in vitro mutagenesis
PCR-based site-directed mutagenesis
In vitro recombination and mutagenesis by overlap extension PCR
Site-directed mutagenesis using overlap extension PCR
Modification of the overlap extension method for extensive mutagenesis on the same template
Site-directed mutagenesis in vitro by megaprimer PCR
Using PCR for rapid site-specific mutagenesis in large plasmids
PCR-assisted mutagenesis for site-directed insertion/deletion of large DNA segments
Site-directed mutagenesis using a rapid PCR-based method
A simple method to introduce internal deletions or mutations into any position of a target DNA sequence
A simple method for site-specific mutagenesis that leaves the rest of the template unaltered
Multiple site-directed mutagenesis
Construction of linker-scanning mutations by oligonucleotide ligation
Construction of linker-scanning mutations using PCR
Use of codon cassette mutagenesis for saturation mutagenesis
Saturation mutagenesis by mutagenic oligonucleotide-directed PCR amplification (mod-PCR)
Random mutagenesis of short target DNA sequences via PCR with degenerate oligonucleotides
Random sequence mutagenesis for the generation of active enzymes
Random mutagenesis by using mixtures of dNTP and dITP in PCR
PCR-mediated chemical mutagenesis
Oligonucleotide-directed random mutagenesis using the phosphorothioate method
An efficient random mutagenesis technique using an E. coli mutator strain