Yeast protocols : methods in cell and molecular biology /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1996]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 53. |
| Subjects: |
Table of Contents:
- Isolation and identification of yeasts from natural habitats
- Maintenance and culture of yeasts
- Mutagenesis in yeast
- Rare-mating and cytoduction in Saccharomyces cerevisiae
- Protoplast fusion in Saccharomyces cerevisiae
- Meiotic analysis
- Ascus dissection
- Karyotyping of yeast strains by pulsed-field gel electrophoresis
- Chromosomal localization of genes through pulsed-field gel electrophoresis techniques
- Isolation, purification and analysis of nuclear DNA in yeast taxonomy
- Isolation of yeast DNA
- Isolation of mitochondrial DNA
- Isolation of yeast plasma membranes
- The extraction and analysis of sterols from yeast
- Peroxisome isolation
- Transformation of lithium-treated yeast cells and the selection of auxotrophic and dominant markers
- Biolistic transformation of yeasts
- Genomic yeast DNA clone banks : construction and gene isolation
- Yeast colony hybridization
- Determination of plasmid copy number in yeast
- Determination of chromosome ploidy in yeast
- Chromosome engineering in yeast with a site-specific recombination system from a heterologous yeast plasmid
- Insertional mutagenesis by Ty elements in Saccharomyces cerevisiae
- Reporter gene systems for assaying gene expression in yeast
- Preparation and use of yeast cell-free translation lysate
- Electrophoretic analysis of yeast proteins
- Preparation of total RNA
- mRNA abundance and half-life measurements
- Polysome analysis
- Induction of heat shock proteins and thermotolerance
- Rhodamine B assay for estimating activity of killer toxins permeabilizing cytoplasmic membranes
- Nystatin-rhodamine B assay for estimating activity of killer toxin from Kluyveromyces lactis
- Application of killer toxins in stepwise selection of hybrids and cybrids obtained by induced protoplast fusion
- Killer plaque technique for selecting hybrids and cybrids obtained by induced protoplast fusion
- Genotoxicity testing in Schizosaccharomyces pombe
- Purification and quantification of saccharomyces cerevisiae cytochrome P450
- Calorimetry of whole yeast cells
- Light microscopy methods
- Fluorescence microscopy methods
- Immunoelectron microscopy