Cover Image

Species diagnostics protocols : PCR and other nucleic acid methods /

Show other versions (1)
Bibliographic Details
Other Authors: Clapp, Justin P.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [1996]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 50.
Subjects:
Polymerase Chain Reaction.
DNA Probes > diagnostic use.
Genetics, Microbial > Laboratory Manuals.
Genetic Techniques.
Table of Contents:
  • Isolation and purification of plant nucleic acids
  • Isolation and purification of insect DNA
  • Isolation and purification of veterbrate DNAs
  • Statistical analysis of arbitrarily primed PCR patterns in molecular taxonomic studies
  • Molecular identification of phytopathogenic viruses
  • Improved PCR methods for identification of phytopathogenic viruses
  • PCR detection of HIV
  • The use of consensus PCR and direct sequence analysis for the identification of HPV
  • The identification of dengue virus using PCR
  • The design and application of ribosomal RNA-targeted, fluorescent oligonucleotide probes for the identification of endosymbionts in protozoa
  • Subtraction hybridization for the isolation of strain-specific Rhizobium DNA probes
  • The use of RAPD for generating specific DNA probes for microorganisms
  • ITS-RFLP matching for identification of fungi
  • Specific PCR primers for the identification of endomycorrhizal fungi.
  • Single-strand conformational polymorphism (SSCP) for the identification of endomycorrhizal fungi.
  • The use of RAPD for isolate identification of arbuscular mycorrhizal fungi
  • Establishing relationships between closely related species using total genomic DNA as a probe
  • Detection and characterization of leishmania parasites by DNA-based methods: southern blotting and PCR
  • The molecular identification of trypanosomes
  • Detection and identification of the four malaria parasite species infecting humans by PCR amplification
  • The use of degenerate primers in conjuction with strain and species oligonucleotides to classify onchocerca volvulus
  • The use of synthetic DNA probes for the field identification of members of the anopheles gambiae complex
  • Restriction fragment length polymorphism (RFLP) analyses: Distinguishing African and European honeybees
  • Differential screening for the isolation of species-specific sequences in the anopheles farauti complex
  • RAPD-PCR with parasitic hymenoptera
  • The use of selective enrichment for the isolation of species-specific DNA probes for insects.