Nonisotopic probing, blotting, and sequencing /

Bibliographic Details
Other Authors:	Kricka, Larry J., 1947-
Format:	Book
Language:	English
Published:	San Diego : Academic Press, [1995]
Edition:	Second edition.
Subjects:	Molecular Probes. Immunoblotting. Luminescence. Amino Acid Sequence. Base Sequence.

Table of Contents:

Introduction
Labels, labeling, analytical strategies, and applications / Larry J. Kricka
Nucleic acid hydridization
Protein blotting
Patents
Methods for nonradioactive labeling of nucleic acids / Christoph Kessler
Methods for enzymatic labeling
Methods for chemical labeling
Methods for chemical labeling of DNA, RNA, and oligodeoxynucleotides with marker enzymes
Overview of factors influencing hybridization
Overview of detection systems
Detection methods
Detection of alkaline phosphatase labels by time-resolved fluorescence / Eva F. Gudgin Dickson, Hector E. Wong, and Alfred Pollak
Detection of alkaline phosphate by bioluminescence / Reinhard Erich Geiger and Werner Miska
Detection of DNA on membranes with alkaline phosphatase-labeled probes and chemiluminescent CSPD R substrate /Annette Tumolo ... et al.
General southern blotting procedure with chemiluminescence
Two-step hybridization southern blotting procedure: detection of single-copy genes
Detection of alkaline phosphatase by colorimetry / Ayoub Rashtchian
Labeling and detection strategies
Hybridization and biotinylated probes
Detection of biotinylated probes
In situ hybridization
Detecion of alkaline phosphatase by chemiluminescence using NADP, ascorbate, and indolyl phosphates / Akio Tsuji, Masako Maeda, and Hidetoshi Arakawa
Detection of horseradish peroxidase by enhanced chemiluminescence / Ian Durrant
Detection of horseradish peroxidase by colorimetry / Peter C. Verlander
Detection of glucose-6-phosphate dehydrogenase by bioluminescence / Jean-Claude Nicolas ... et al.
Detection of xanthine oxidase by enhanced chemiluminescence / Alain Baret.
Electrochemiluminescent detection of PCR-derived nucleic acids / Elena D. Katz and John M. Wages
Detection of phosphors by phosphorescence /H. Berna Beverloo
Detection of europium cryptates by time-resolved fluorescence / Odette Prat, Evelyne Lopez, and Gerard Mathis
Detection of lanthanide chelates by time-resolved fluorescence / Timo Lovgren ... et al
Indirect labeling
Chemical europium labeling of DNA probes
Enzymatic europium labeling of DNA probes
Europium-labeled oligonucleotides
Detection of mutation using lanthanide-labeled oligonucleotides
Detection of lanthanide chelates and multiple labeling strategies based on time-resolved fluorescence / Eleftherios P. Diamandis and Theodore K. Chroistopoulos
Detection of acridinium esters by chemiluminescence / Norman C. Nelson, Mark A. Reynolds, and Lyle J. Arnold.
Detection of energy transfer and fluorescence quenching / Larry E. Morrison.
DNA sequencing / Parke K. Flick
Automated fluorescent detection of DNA sequences
Manual nonisotopic detection of DNA sequences
Future developments in nonisotopic DNA sequencing
Chemiluminescent DNA sequencing with 1,2-dioxetanes / Chris S. Martin and Irena Bronstein
Chemiluminescent DNA sequencing procedure using biotinylated primers
Detection of biotinylated DNA sequencing reactions with streptavidin and biotinylated alkaline phosphatase
Chemiluminescent DNA sequencing with hapten-labeling primers and detection with antibody-alkaline phosphatase conjugates.