Yeast gene analysis /

Bibliographic Details
Other Authors: Brown, Alistair J. P., 1955-, Tuite, Mick F., 1953-
Format: Book
Language:English
Published: San Diego, Calif. : Academic Press, [1998]
Series:Methods in microbiology. v. 26.
Subjects:
Table of Contents:
  • Introduction to functional analysis of the yeast genome
  • Whole chromosome analysis
  • The bioinformatics of the yeast genome
  • Transformation of yeast by the lithium acetate/single-stranded carrier DNA/PEG method
  • PCR-based gene targeting in Saccharomyces cerevisiae
  • Studying essential genes: generating and using promoter fusions and conditional alleles
  • Ty mutagenesis
  • Transcript analysis
  • Reporter genes and their use in studying yeast gene expression
  • Transposon tagging I: a novel system for monitoring protein production, function and localization
  • Transposon ragging II: exploration of gene function and regulatory networks in yeast with the mini-mu transposon
  • Immunological approaches to the study of protein localization in yeast
  • Posttranslational modifications of secretory proteins
  • Studying posttranslational modifications in yeast: intracellular proteins
  • Two-hybrid analysis of protein-protein interactions in yeast
  • "Smart" genetic screens
  • Metabolic control analysis as a tool in the education of the function of novel genes
  • Identifying stress genes
  • Identifying genes encoding components of the protein synthesis machinery of the yest Saccharomyces cerevisiae
  • MAP kinase-mediated signal transduction pathways
  • Analysis of the Candida albicans genome
  • Applications of yeast in the discovery of human gene function
  • Yeast mutant and plasmid collections
  • Yeast gene analysis in the next millennium.