Ribozyme protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1997]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 74. |
| Subjects: |
Table of Contents:
- Ribozymes : an introduction
- Computer-aided calculation of the local folding potential of target RNA and its use for ribozyme design
- Computational approaches to the identification of ribozyme target sites
- Computer analysis of the conservation and uniqueness of ribozyme-targeted HIV sequences
- Selection of accessible sites for ribozymes of large RNA transcripts
- Selection of efficient ribozyme cleavage sites in target RNAs
- Chemical synthesis, analysis and purification of ribozymes
- A practical method for the production of RNA and ribozymes
- Preparation of templates for production of ribozymes and substrates
- Enzymatic synthesis and characterization of unmodified ribozymes and substrates
- T7 transcript length determination usng enzymatic RNA sequencing
- Chemical and enzymatic approaches to construct modified RNAs
- Applications of modified transcipts
- Cloning strategies for catalytic antisense RNAs
- PCR-based construction of long hammerhead ribozymes
- Design and production of asymmetric hammerhead ribozymes
- Minimized hammerhead ribozymes
- Design of hairpin ribozymes for invitro and cellular applications
- Dsign of the hairpin ribozyme for targeting specific RNA sequences
- Design and preparation of sequence-specific RNase P ribozymes
- Theoretical considerations in measuring reaction parameters
- Experimental approaches for measuring reaction parameters
- Determination of catalytic parameters for hairpin ribozymes
- Characterizing ribozyme cleavage reactions
- Defining optimum reaction conditions for hammerhead ribozymes
- Using fluorescence resonance energy transfer to investigate hammerhead ribozyme kinetics
- Design of hybridizing arms in hammerhead ribozymes
- Optimization of hammerhead flanking sequences using oligonucleotide facilitators
- Enhancement of ribozyme function by RNA binding proteins
- Selection of fast-hybridizing complementary RNA species in vitro
- In vitro selection of hairpin ribozymes
- The detection of hammerhead ribozyme cleavage by RT-PCR methods
- Detection of ribozyme cleavage products using reverse ligation-mediated PCR (RL-PCR)
- Quantitation of ribozyme target abundance by QCPCR
- Trans-splicing reactions by ribozymes
- Ligation of RNA molecules by the hairpin ribozyme
- Mutagenesis and modeling of the hairpin ribozyme family
- Preparation of homogenous ribozyme RNA for crystallization
- Establishing suitability of RNA preparations for crystallization : determination of polydispersity
- A sparse matrix approach to crystallizing ribozymes and RNA motifs
- Crystallographic analyses of chemically synthesized modified hammerhead RNA sequences as a general approach toward understanding ribozyme structure and function
- tRNA delivery systems for ribozymes
- Expressing ribozymes in plants
- Using microinjection of Xenopus oocytes to express and optimize ribozymes in vivo
- Exogenous cellular delivery of ribozymes and ribozyme encoding DNAs
- Optimization of lipid-mediated ribozyme delivery to cells to culture
- Retroviral delivery and anti-HIV testing of hammerhead ribozymes
- Hairpin ribozyme gene therapy for AIDS
- Clinical aspects of ribozymes as therapeutics in gene therapy