http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
xxiv, 486 pages : illustrations ; 29 cm.
Introduction -- Biology of the cultured cell -- Design and layout of the laboratory -- Equipping the laboratory -- Aseptic technique -- Laboratory safety and biohazards -- Culture environment: substrate, gas phase, medium and temperature -- Preparation and sterilization -- Disaggregation of the tissue and primary culture -- Maintenance of the culture: cell lines -- Cloning and selection of specific cell types -- Physical methods of cell separation -- Characterization -- Induction of differentiation -- Transformed phenotype -- Contamination -- Instability, variation and preservation -- Quantitation and experimental design -- Measurement of viability and cytotoxicity -- Culture of specific cell types -- Culture of tumor tissue -- Three-dimensional culture systems -- Specialized techniques.
Includes bibliographical references and index.