Methods and techniques in virology /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York :
Marcel Dekker,
[1993]
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| Subjects: |
Table of Contents:
- Laboratory safety
- Types of containment
- Monolayer cell culture
- Cell culture media
- Maintenance of cell cultures
- Cell cloning
- Colony formation test
- Cryopreservation
- Chromosome preparations
- Isolation and identification of viruses
- Preparation of specimens
- Inoculation to embryonated eggs
- Isolation of viruses in cell cultures
- Identification of viruses
- Animal models
- Major species
- Factors influencing the choice and use of animals
- Visible signs
- Inoculation of laboratory animals
- Supplies
- Methods
- Concentration of viruses
- Methods
- Applications
- Protein assays
- Spectrophotometric method
- Colorimetric method
- Ultracentrifugation
- Differential centrifugation
- Density gradient centrifugation
- Parameters of ultracentrifugation
- Chromatography
- Gel filtration
- Ion-exchange chromatography
- Affinity chromatography
- Electrophoresis
- Electron microscopy
- Specimen fixation
- Negative staining
- Serology
- Supplies
- Hemadsorption: inhibition test
- Hemagglutination
- Hemagglutination inhibition (HI) test.
- (cont) Neutralization test
- Immunoassays and detection of specific classes of immunoglobulins
- Immunofluorescence
- Preparation of material
- Methods
- Microscopy
- Immunoperoxidase
- Light microscopy
- PAP and ABC methods
- ELISA
- Antibody detection
- Monoclonal antibodies: identification of antigenic sites
- Competition assay
- Selection of antigenic variants
- Histology and histochemistry
- Tissue fixation
- Impregnation
- Embedding
- Cryostat sections
- Sections staining
- Staining of cells in wells and on coverslips
- Exfoliative cytology
- Mounting
- Immunocytochemical staining in electron microscopy
- Ferritin
- Horseradish peroxidase
- Colliodal gold
- Metabolic and chemical radiolabeling
- Nucleic acids
- Proteins
- Polysaccharides
- Lipids
- Identification of viral proteins by immunoprecipitation
- Identification of viral proteins by western blotting
- Peptide mapping
- Protein separation in polyacrylamide gels
- Chemical cleavage
- Agarose gel electrophoresis of DNA molecules
- Genotyping
- Extraction of viral nucleic acids.
- (cont) In vitro translation
- In vitro translation systems
- Cloning of genes from DNA viruses
- Synthesis of complementary DNA
- Synthesis of complementary DNA from single-stranded RNA
- Spreading and visualization of nucleic acid molecules in electron microscopy
- Material
- Transfer techniques
- Contrasting the nucleic acids
- Molecular weight determinations
- Molecular hybridization
- In situ hybridization of bacterial colony
- Southern blot hybridization
- Northern blotting
- Hybridization
- Detection of hybrids and reuse of filters
- Virus detection by hybridization
- Polymerase chain reaction
- General principles
- Solutions
- Method.