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PCR applications : protocols for functional genomics /

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Bibliographic Details
Other Authors: Sninsky, John J., Innis, Michael A., Gelfand, David H.
Format: Book
Language:English
Published: San Diego ; London : Academic Press, [1999]
Subjects:
Polymerase Chain Reaction > methods > Laboratory Manuals.
Genetic Engineering > Laboratory Manuals.
Table of Contents:
  • Pt. 1. Key concepts for PCR
  • Optimization of PCR: conversations between Michael and David
  • The Convergence of PCR, computers, and the human genome project: past, present, and future
  • Thermostable DNA polymerases: an update
  • Musings on microbial genomes
  • Statistical refinement of primer design parameters
  • Multiplex PCR: optimization guidelines
  • The Use of immobilized mismatch binding protein for the optimization of PCR fidelity.
  • A New generation of PCR instruments and nucleic acid concentration systems
  • Sequencing PCR products
  • Recent advances in high-temperature reverse transcription and PCR
  • Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance
  • In situ PCR
  • Pt. 2. Quantitative PCR
  • Standards for PCR assays
  • Rapid thermal cycling and PCR kinetics
  • Kinetics of competitive reverse transcriptase-PCR.
  • Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes
  • Quantification of telomerase activity using telomeric repeat amplification protocol
  • Gene discovery
  • Differential display
  • Single-cell cDNA libraries
  • Whole cell assays
  • Screening differentially displayed PCR products by single-strand conformation polymorphism gels
  • Microsatellite protocols
  • Real-time quantitative PCR: uses in discovery research.
  • Homology cloning: a molecular taxonomy of the archaea
  • Cloning mammalian homologs of drosophila genes
  • Cloning human homologs of yeast genes
  • Pt. 4. Genomics and expression profiling
  • Cellular transcriptome analysis using a kinetic PCR assay
  • Parallel analysis with biological chips
  • High-density cDNA grids for hybridization fingerprinting experiments
  • Comparative genomics hybridization
  • Genetic footprinting and functional maps of the yeast genome.
  • Molecular analysis of microdissected tissue: laser capture microdissection
  • Amplified fragmant length polmorphism: studies on plant development
  • A Florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes
  • The Cleavase I enzyme for mutation and polymorphism scanning.