rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Handbook of detection of enzymes on electrophoretic gels
  name:
    name part: Manchenko, Gennady P.
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: flu
    date issued: 1994
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: 341 pages : illustrations ; 29 cm.
  table of contents: General principles of enzyme detection on electrophoretic gels -- Chromogenic reactions -- Products reducing tetrazolium salts -- Products capable of coupling with diazonium salts -- Products that cause a pH change -- Orthophosphate -- Pyrophosphate -- Hydrogen peroxide -- Carbonate ions -- Colored products -- Products bearing reduced thiol groups -- Products that influence the starch-iodine reaction -- Products of polymerizing enzymes -- Products of depolymerizing enzymes -- Fluorogenic reactions -- NADH and NADPH -- 4-methylumbelliferone.
  table of contents: Products that form luminescent lanthanide chelates -- Miscellany -- Autoradiography -- Bioautography -- Two-dimensional gel spectroscopy -- Immunoblotting -- Immobilization matrices -- Transfer of proteins -- Specific antibodies and labeled anti-antibodies -- Detection of transferred proteins on blots -- Methods of detection of specific enzymes -- Structure of enzyme sheets -- General considerations, comments and recommendations -- Choice of support medium for enzyme electrophoresis and detection -- Cellulose acetate gel -- Polyacrylamide gel.
  table of contents: Starch gel -- Strategies of gel staining -- Choice of zymogram method -- Preparation of staining solution -- Modes of application of staining solutions -- Modes of enhancement of staining intensity of enzyme activity bands -- Specificity of zymogram methods and some related problems -- Recording and preservation of zymograms -- Resource-saving strategies -- Simultaneous detection of several enzymes on the same gel -- Successive detection of different enzymes on the same gel -- Reusing of staining solutions -- Use of several origins on one gel.
  table of contents: Multiple replication of electrophoretic gels by electroblotting -- Use of semipreparative gels for production of preparations of linking enzymes -- Troubleshooting -- Safety regulations -- Enzyme sheets -- Minimal medium for Escherichia coli -- Citrate medium for Pediococcus cerevisiae -- Alphabetical list of enzymes.
  note: Gennady P. Manchenko.
  note: Includes bibliographical references.
  subject:
    topic: Enzymes
    topic: isolation & purification
    genre: Laboratory Manuals
  subject:
    topic: Electrophoresis, Polyacrylamide Gel
    genre: Laboratory Manuals
  classification: QP601 .M314 1994
  classification: 574.19/25
  classification: 1994 I-703
  classification: QU 25 M268h 1994
  identifier: 0849389356
  identifier: 94011112
  record info:
    record content source: DLC
    record creation date: 940328
    record change date: 20210818135149.0
    record identifier: in00004481591