http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Handbook of detection of enzymes on electrophoretic gels
General principles of enzyme detection on electrophoretic gels -- Chromogenic reactions -- Products reducing tetrazolium salts -- Products capable of coupling with diazonium salts -- Products that cause a pH change -- Orthophosphate -- Pyrophosphate -- Hydrogen peroxide -- Carbonate ions -- Colored products -- Products bearing reduced thiol groups -- Products that influence the starch-iodine reaction -- Products of polymerizing enzymes -- Products of depolymerizing enzymes -- Fluorogenic reactions -- NADH and NADPH -- 4-methylumbelliferone.
Products that form luminescent lanthanide chelates -- Miscellany -- Autoradiography -- Bioautography -- Two-dimensional gel spectroscopy -- Immunoblotting -- Immobilization matrices -- Transfer of proteins -- Specific antibodies and labeled anti-antibodies -- Detection of transferred proteins on blots -- Methods of detection of specific enzymes -- Structure of enzyme sheets -- General considerations, comments and recommendations -- Choice of support medium for enzyme electrophoresis and detection -- Cellulose acetate gel -- Polyacrylamide gel.
Starch gel -- Strategies of gel staining -- Choice of zymogram method -- Preparation of staining solution -- Modes of application of staining solutions -- Modes of enhancement of staining intensity of enzyme activity bands -- Specificity of zymogram methods and some related problems -- Recording and preservation of zymograms -- Resource-saving strategies -- Simultaneous detection of several enzymes on the same gel -- Successive detection of different enzymes on the same gel -- Reusing of staining solutions -- Use of several origins on one gel.
Multiple replication of electrophoretic gels by electroblotting -- Use of semipreparative gels for production of preparations of linking enzymes -- Troubleshooting -- Safety regulations -- Enzyme sheets -- Minimal medium for Escherichia coli -- Citrate medium for Pediococcus cerevisiae -- Alphabetical list of enzymes.