http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
xvii, 462 pages : illustrations ; 24 cm.
Introduction -- Electrophoresis -- Microchromatographic separation methods -- Capillary electrophoresis -- Capillary electrochromatography -- Micromachined electrophoretic devices -- Historical perspective -- Generic HPCE systems -- Instrumentation -- Sources of information on HPCE -- Capillary electrophoresis: a family of techniques -- Capillary zone electrophoresis: basic concepts -- Electrical conduction in fluid solution -- The language of electrophoresis -- Electroendoosmosis -- Efficiency -- Resolution -- Joule heating -- Optimizing the voltage and temperature -- Capillary diameter and buffer ionic strength -- Optimizing the capillary length -- Buffers -- Temperature effects -- Buffer additives -- Capillaries -- Sources of bandbroadening -- Capillary zone electrophoresis: methods development -- Introduction -- Mobility -- Solute-wall interactions -- Separation strategies -- Secondary equilibrium -- Applications and techniques -- Capillary zone electrophoresis: secondary equilibrium, micelles, cyclodextrins, and related reagents -- Micelles -- Separation mechanism -- Selecting the electrolyte system -- Elution range of MECC -- Alternative surfactant systems -- Cyclodextrins -- Applications and methods development -- Chiral recognition -- Affinity capillary electrophoresis -- Capillary isoelectric focusing -- Basic concepts -- Separation mechanism -- pH gradient formation -- Electrode buffer solutions -- Resolving power -- Capillaries and reagents -- Performing a run -- Injection -- Focusing -- Mobilization -- Salt effects -- Detection -- Applications -- Size separations in capillary gels and polymer networks -- Separation mechanism -- Materials for size separations -- Size separations with nonreplaceable polyacrylamide -- Size separations with replaceable agarose -- Introduction to polymer networks -- Operating characterstics of polymer networks -- Detection -- Operating hints using polymer networks -- Applications and methods development -- Reducing the problem of biased reptation -- Capillary electrochromatography -- Modes of CEC -- Electroosmotic flow in CEC -- Efficiency of CEC -- Operating characteristics of packed CEC -- Applications -- CEC microfluidic devices -- Injection -- Volumetric constraints on injection size -- Performing an injection and a run -- Injection techniques -- Short-end injection -- Injection artifacts: problems and solutions -- Stacking and trace enrichment -- Detection -- On-capillary detection -- The detection problem -- Limits of detection -- Detection techniques -- Band broadening -- Absorption detection -- Fluorescence detection -- Derivatization -- Mass spectrometry -- Micropreparative fraction collection -- Putting it all together -- Selecting the mode of HPCE -- Requirements for robust separations -- Realistic compromises -- Quantitative analysis -- Sample preparation -- Mobility as a qualitative tool -- Validation -- Troubleshooting.
Includes bibliographical references and index.