http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Introduction to the principles of flow cytometry / Nigel P. Carter and Michael G. Ormerod -- Introduction -- Techniques for sample preparation -- Fluidics -- Detection and measurement -- Cell sorting -- Fluorescence and fluorochromes / Michael G. Ormerod -- Introduction -- Principles -- Fluorophores used to label covalently other probes -- Fluorophores used to label directly cell components -- Preparing suspensions of single cells / Michael G. Ormerod -- Introduction -- Cells from peripheral blood -- Leucocytes from other tissue -- Other types of cell -- Cultured cells -- Flow sorting / Nigel P. Carter and Michael G. Ormerod -- Introduction -- Principles of flow sorting -- Parameters which affect sorting -- Practical considerations -- Specialized features -- Other flow-sorting systems -- Immunofluorescence of surface markers / Michael R. Loken, Cherie L. Green and Denise A. Wells -- Introduction -- Instrument standardization -- Testing sensitivity and resolution -- Immunofluorescence -- Staining for surface markers -- Titration of antibodies -- CD nomenclature -- Population assignment -- Constructing an immunophenotyping panel -- Isotype controls -- DNA content/surface marker analysis -- Analysis of DNA - general methods / Michael G. Ormerod -- Introduction -- Definitions -- General comments -- Experimental methods -- Deconvolution of the DNA histogram -- Further clinical applications / Terry Hoy ... [et al.] -- Introduction -- The enumeration of reticulocytes in peripheral blood -- The enumeration of CD34+ cells in peripheral blood stem-cell harvests and bone-marrow harvests -- Simultaneous detection of granulocyte- and lymphocyte-reactive antibodies -- Detection of platelet-reactive antibodies -- Application of crossmatching by flow cytometry to transplantation -- Monitoring anti-thymocyte globulin (ATG)/anti-lymphocyte globulin (ALG) therapy in transplantation -- Measurement of cell-mediated cytotoxicity by flow cytometry -- Monitoring cell activation in response to antigens or mitogens -- Measuring intracellular cytokines by flow cytometry -- Quality assurance in the clinical laboratory / Jan W. Gratama and David Barnett -- Introduction -- Reference materials for calibration -- Internal quality-control procedures -- External quality assurance -- Measurement of cytoplasmic and nuclear antigens / Jørgen K. Larsen -- Introduction -- Methods of cell permeabilization -- Methods of cell staining and analysis -- Examples of intracellular antigens that can be measured by flow cytometry -- Analysis of DNA - measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method / George D. Wilson -- Introduction -- Basic cell-kinetic concepts -- Background to the BrdUrd technique -- Methods of BrdUrd incorporation -- Tissue preparation -- Staining procedures -- Flow cytometry -- Examples of data and analysis -- Conclusions -- Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-ethidium bromide method / Michael G. Ormerod and Martin Poot -- Introduction -- Cell culture and BrdUrd labelling -- Cell staining and flow cytometry -- Synchronized cells -- Asynchronous cells -- Troubleshooting -- Chromosome analysis and sorting by flow cytometry / Derek C. Davies, Simon P. Monard, and Bryan D. Young -- Introduction -- Preparation of chromosome suspensions -- Instrumentation -- Flow sorting chromosomes for library construction -- Generation of chromosome paints -- Intracellular ionized calcium, magnesium, membrane potential, and pH / Peter S. Rabinovitch and Carl H. June -- Intracellular ionized calcium -- Magnesium -- Membrane potential -- Measurement of cytoplasmic pH -- Flow cytometry in the study of apoptosis / Michael G. Ormerod -- Introduction -- Morphological and biochemical changes -- Measurement of DNA degradation -- Mitochondrial membrane potential -- Measurements of changes in the plasma membrane -- Other measurements -- Quantification of apoptotic cells -- Further applications to cell biology / Michael G. Ormerod -- Introduction -- Estimating cell viability -- Monitoring electropermeabilization of cells -- Measurement of oxidative burst -- Characterizing multi-drug resistance (MDR) in cancer cells -- Safety procedures -- Bench-top flow cytometers -- Cell sorters -- Chemicals -- Flow cytometers and software -- Commercial flow cytometers -- Software for data manipulation -- List of suppliers.
Includes bibliographical references and index.