Extracellular matrix protocols /

Bibliographic Details
Other Authors: Streuli, Charles H., Grant, Michael E.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2000]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 139.
Subjects:
Table of Contents:
  • Pt. 1. Biochemistry of extracellular matrix
  • Semipermeabilized cells to study procollagen assembly / Richard R. Wilson, Neil J. Bulleid
  • Quantitative determination of collagen crosslinks / Trevor J. Sims, Nicholas C. Avery, Allen J. Bailey
  • Analysis of laminin structure and function with recombinant glycoprotein expressed in insect cells / Todd L. Mathus, Peter D. Yurchenco
  • Recombinant collagen trimers from insect cells and yeast / Johanna Myllyharju
  • Eukaryotic expression and purification of recombinant extracellular matrix proteins carrying the strep II tag / Neil Smyth ... [et al.]
  • Pt. 2. Biophysics of extracellular matrix proteins
  • Preparation of isotopically labeled recombinant fragments of fibronectin for functional and structural study by heteronuclear nuclear magnetic resonance spectroscopy / Jeremy R. Bright ... [et al.]
  • Reconstitution of functional integrin into phopsholipid vesicles and planar lipid bilayers / Eva-Maria Erb, Jürgen Engel
  • Confocal-FRAP analysis of ECM molecular interactions / Timothy Hardingham, Philip Gribbon
  • Electron cryomicroscopy of fibrillar collagens / Roger S. Meadows ... [et al.]
  • Atomic force microscopy measurements of intermolecular binding strength / Gradimir N. Misevic
  • ECM macromolecules: rotary shadowing and scanning transmission electron microscopy / Michael J. Sherratt ... [et al.]
  • Pt. 3. Molecular biology of extracellular matrix genes
  • Screening for mutations in cartilage ECM genes / Michael D. Briggs
  • Tissue-specific KO of ECM proteins / Emilio Hirsch, Mara Brancaccio, Fiorella Altruda
  • Homologous gene targeting to study ECM assembly / Francesco Ramirez, Friedrich Laub, Hideaki Sumiyoshi
  • Enhancer analysis of the α1(II) and α2(XI) collagen genes in transfected chondrocytes and transgenic mice / Noriyuki Tsumaki ... [et al.]
  • Retroviral delivery of ECM genes to cells / Kyonggeun Yoon, Vitali Alexeev
  • Pt. 4. Cell biology of the extracellular matrix
  • Using self-assembled monolayers to pattern ECM proteins and cells on subtrates / Christopher S. Chen ... [et al.]
  • Methods for preparing extracellular matrix and quantifying insulin-like growth factor-binding protein binding to the ECM / Bo Zhen, David R. Clemmons
  • Measuring interactions between ECM and TGFβ-like proteins / Sarah L. Dallas
  • Using organotypic tissue slices as substrata for the culture of dissociated cells / Daniel E. Emerling, Arthur D. Lander
  • Neuroepithelial differentiation induced by ECM molecules / José María Frade, Alfre Rodriguez Tébar
  • Migration assays for oligodendrocyte precursor cells / Emma E. Frost, Richard Milner, Charles ffrench-Constant
  • Cell adhesion assays / Martin J. Humphries
  • Tissue engineering and cell-populated collagen matrices / Paul D. Kemp
  • Solid phase assays for studying ECM protein-protein interactions / A. Paul Mould
  • Tissue engineering of cartilage / Ronda E. Schreiber, Anthony Ratcliffe
  • Tissue recombinants to study extracellular matrix targeting to basement membranes / Patricia Simon-Assmann, Michèle Kedinger
  • Fluorescence assays to study cell adhesion and migration in vitro / Paola Spessotto, Emiliana Giacomello, Roberto Perris
  • Analyzing cell-ECM interactions in adult mammary gland by transplantation of embryonic mammary tissue from knockout mice / Teresa C.M. Klinowska, Charles H. Streuli.