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Developmental biology protocols /

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Bibliographic Details
Other Authors: Tuan, Rocky S., Lo, Cecilia W.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2000]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 135.
Methods in molecular biology (Clifton, N.J.) ; v. 136.
Methods in molecular biology (Clifton, N.J.) ; v. 137.
Subjects:
Developmental Biology.
Molecular Biology.
Laboratory Manual.
Table of Contents:
  • Vol. 1. (v.135)
  • Developmental biology protocols : overview I / R.S. Tuan ... [et al.]
  • Rearing larvae of sea urchins and sea stars for developmental studies / C.J. Lowe ... [et al.]
  • Large-scale culture and preparation of sea urchin embryos for isolation of transcriptional regulatory proteins / J.A. Coffman ... [et al.]
  • The chick embryo as a model system for analyzing mechanisms of development / D.K. Darnell ... [et al.]
  • Culture of avian embryos / D.K. Darnell ... [et al.]
  • Ex ovo culture of avian embryos / T. Ono
  • Culture of preimplantation mouse embryos / A.S. Doherty ... [et al.]
  • In vitro culture of rodent embryos during early postimplantation period / M. Fujinaga
  • Cryopreservation of mouse embryos / J. Richa
  • Studying head and brain development in Drosophila / R. Finkelstein
  • Bioassays of inductive interactions of amphibian development / T. Ariizumi ... [et al.]
  • Gastrulation and early mesodermal patterning in vertebrates / G.C. Schoenwolf ... [et al.]
  • Craniofacial development of avian and rodent embryos / B.K. Hall ... [et al.]
  • Examination of the axial skeleton of fetal rodents / M.G. Narotsky ... [et al.]
  • Cardiac morphogenesis and dysmorphogenesis : an immunohistochemical approach / B.R. Waller III ... [et al.]
  • Application of plastic embedding for sectioning whole-mount immunostained early vertebrate embryos / K.K. Linask ... [et al.]
  • Confocal microscopy of live Xenopus oocytes, eggs, and embryos / C.A. Larabell
  • Whole-mount immunolabeling of embryos by microinjection : increased detection levels of extracellular and sell surface epitopes / C.J. Drake ... [et al.]
  • Confocal laser scanning microscopy of morphology and apoptosis in organogenesis-stage mouse embryos / R.M. Zucker ... [et al.]
  • Embryo/fetal topographical analysis by fluorescence microscopy and confocal laser scanning microscopy / R.M. Zucker ... [et al.]
  • Magnetic resonance imaging analysis of embryos / B.R. Smith
  • Optical coherence tomography imaging in developmental biology / S.A. Boppart ... [et al.]
  • Ultrasound backscatter microscopy of mouse embryos / D.H. Turnbull
  • Use of Doppler echocardiography to monitor embryonic mouse heart function / K.K. Linask ... [et al.]
  • Calcium imaging and cell-cell signaling / D. Slusarski ... [et al.]
  • Acquisition display and analysis of digital 3D and time-lapse (4D) data sets using free software applications / C.F. Thomas ... [et al.]
  • Cell lineage analysis : applications of green fluorescent protein / M. Zernicka-Goetz ... [et al.]
  • Cell lineage analysis : X-inactivation mosaics / S.-S. Tan ... [et al.]
  • Retroviral cell lineage analysis in the developing chick heart / R.G. Gourdie ... [et al.]
  • Dynamic labeling techniques for fate mapping testing cell commitment and following living cells in avian embryos / D.K. Darnell ... [et al.]
  • Cell lineage analysis : videomicroscopy techniques / P.J. Heid ... [et al.]
  • Cell lineage analysis in Xenopus embryos / S.A. Moody
  • Photoactivatable(caged) fluorescein as tracer for fate mapping in the zebrafish embryos / D.J. Kozlowski ... [et al.]
  • Carboxyfluorescein as a marker at both light and electron microscope levels to follow cell lineage in the embryo / D. Sun ... [et al.]
  • Transformation chimeras : use in analyzing mechanisms of avian development / D.K. Darnell ... [et al.]
  • Interspecific chimeras in avian embryos / N.M. LeDouarin ... [et al.]
  • Quail-chick transplantation in the embryonic limb bud / E.E. LeClair ... [et al.]
  • Mouse chimeras and the analysis of development / R. Gardner ... [et al.]
  • Cell grafting and fate mapping of the early-somite-stage-mouse embryo / S.J. Kider ... [et al.]
  • Interspecific chimeras : transplantation of neural crest between mouse and chick embryos / M.L. Kirby ... [et al.]
  • Interspecific mouse-chick chimeras / J. Fontaine-Perus
  • Mosaic analysis in Caenorhabdiris elegans / J. Yochem ... [et al.]
  • Local application of BMP on developing chick embryos using a fibrous glass matrix as carrier / A. Nifuji ... [et al.]
  • Laser ablation and fate mapping / M.L. Kirby ... [et al.]
  • Photoablation of cells expressing beta-glasctosidase / S. Nirenberg
  • Exo utero surgery / K. Muneoka ... [et al.]
  • Methods for constructing and producing retroviral vectors / A. Gambotto ... [et al.]
  • Retroviral gene transduction in limb bud micromass cultures / N.S. Scott ... [et al.]
  • Construction of adenoviral vectors / A.R. Davis ... [et al.]
  • Application of adenoviral vectors : analysis of eye development / J. Bennett ... [et al.]
  • Application of adenoviral vectors in the study of mammalian cardiovascular development / C.S. Mickanin ... [et al.]
  • Vol. II. (v.136)
  • Developmental biology protocols : overview II / R.S. Tuan ... [et al.]
  • Drosophila as a genetic tool to define vertebrate pathways players / N.M. Bonnie
  • Bioassays for studying the role of the peptide growth factor actin in early amphibian embryogenesis / M. Asashima ... [et al.]
  • Analysis of mammary gland morphogenesis / C. D. Roskelley ... [et al.]
  • Specification of cardiac mesenchyme and heart morphogenesis in vitro / H.J. Yost
  • Craniofacial development and patterning / H. Slavkin ... [et al.]
  • Craniofacial skeletal morphogenesis in vitro / R.C. Ogle
  • Skeletal morphogenesis / S. Mundlos
  • Transplantation and culture techniques for the analysis of urodele limb regeneration / D.L. Stocum
  • Retroviral infection of T-cell precursors in thymic organ culture / L.M. Spain ... [et al.]
  • Assay for the isolation of hepatogenic factors : key molecules in hepatocyte formation / J.A.A. Spijkers ... [et al.]
  • Skin morphogenesis : embryonic chicken skin explant cultures / C.-M. Chuong
  • Apoptosis in development / L. Lin ... [et al.]
  • Methods to detect patterns of cell death in Drosophila / N.M. Bonini
  • Mechanisms of neurulation / G.C. Schoenwolf ... [et al.]
  • Neurulation and neural tube closure defects / A. Copp ... [et al.]
  • Neural tube defects / T. Inagaki ... [et al.]
  • Experimental manipulation and morphometric analysis of neural tube development / M.E. Desmond ... [et al.]
  • Isolation of neuroepithelium and formation of minispheres / M.E. Desmond ... [et al.]
  • Examination of normal and abnormal placentation in the mouse / M.R. Blackburn
  • Palatal dysmorphogenesis : quantitative RT-PCR / G.A. Held ... [et al.]
  • Transspecies grafting as a tool to understand the basis of murine development limb abnormalities / S.M. Bell ... [et al.]
  • Assessment of laterality defects in rodent embryos / M. Fujinaga
  • Cardiac morphogenesis and dysmorphogenesis : I. normal development / A. Wessels ... [et al.]
  • Analysis of two aspects of left-right patterning of the vertebrate heart : heart tube position and heart tube chirality / A. Chin
  • Biologically based risk assessment models for developmental toxicity / C. Lau ... [et al.]
  • Positional cloning / L. Xu ... [et al.]
  • Gene trapping in embryonic stem cells in vitro to identify novel developmentally regulated genes in the mouse / G.E. Lyons ... [et al.]
  • PCR-based cloning of cortically localized RNA from Xenopus oocytes / J. Zhang ... [et al.]
  • Analysis of mRNA expression during preimplantation development / K.E. Latham ... [et al.]
  • Differential screens with subtracted PCR-generated cDNA libraries from subregions of single mouse embryos / A. Shimono ... [et al.]
  • HPLC-based mRNA differential display / T.B. Knudsen
  • Production of transgenic Drosophila / M. Fujioka ... [et al.]
  • Transgenic manipulation of the sea urchin embryo / J.P. Rast
  • Transgenic zebrafish / S. Lin
  • Production of avian chimera and germline transmission / K.L. Woods ... [et al.]
  • Incorporation of genetically modified cells into chicken chimeras / L.D. Zajchowski ... [et al.]
  • Long-term culture of chicken blastodermal cells (CBCs) and selection of transfected CBCs using antibiotic resistance / Q. Wei ... [et al.]
  • Nuclear transplantation and cloning in mammals / K.E. Latham ... [et al.]
  • Production of transgenic mice / J. Richa
  • Production of transgenic mice with yeast artificial chromosome / A. Jakobovits ... [et al.]
  • Gene-targeting strategies / S.-S. Cheah ... [et al.]
  • Chimeric animals and germline transmission / J.S. Khillan
  • Conditional gene knockout using Cre recombinase / Y. Le ... [et al.]
  • Application of Cre/IoxP in Drosophila : site-specific recombination and transgene coplacement / M.L. Siegal ... [et al.]
  • Vol. III. (v.137)
  • Developmental biology protocols : overview III / R.B. Tuan ... [et al.]
  • Ectopic expression in Drosophila / E.L. Wilder
  • Clonal analysis in the examination of gene function in Drosophila / J.E. Rooke ... [et al.]
  • Application of antisense oligodeoxynucleotides in developing chick embryos / P.G. Alexander ... [et al.]
  • Application of functional blocking antibodies : N-cadherin and chick embryonic limb development / S.A. Oberlender ... [et al.]
  • Gene expression analyzed by ribonuclease protection assay / V.D. Bennett
  • Relative reverse transcription-polymerase chain reaction / T.A. Giambernardi ... [et al.]
  • Gene expression analysis using quantitative reverse transcription-polymerase chain reaction and a multispecific internal control / D. Shire ... [et al.]
  • In situ PCR detection of HIV expression in the human placenta / A.U. Sheikh ... [et al.]
  • Gene expression analysis by in situ hybridization : radioactive probes / S. Wawersik ... [et al.]
  • Radio-isotopic in situ hybridization on tissue sections : practical aspects and quantification / A.F.M. Moorman ... [et al.]
  • mRNA and protein co-localization on tissue sections by sequential, colorimetric in situ hybridization and immunohistochemistry / R.S. Tuan
  • Whole-mount in situ hybridization to study gene expression during mouse development / L.A. Lowe ... [et al.]
  • Multicolor whole-mount in situ hybridization / G. Hauptmann ... [et al.]
  • Methods for double detection of gene expression : combined in situ hybridization and immunocytochemistry or histochemistry / R.A. Conlon
  • Visualization of the expression of green fluorescent protein (FTP)-linked proteins / J.C. Ayoob ... [et al.]
  • Monoclonal antibodies in the analysis of embryonic development / V. Bedian
  • Mesoderm induction in Xenopus : oocyte expression system and animal cap assay / J. Yao ... [et al.]
  • Amphibian organizer activity / SC. Niehrs
  • Improved techniques for avian embryo culture, somite cell culture, and microsurgery / D.S. Packard, Jr. ... [et al.]
  • Neural crest cell outgrowth cultures and the analysis of cell migration / D.F. Newgreen ... [et al.]
  • The chimeric human/mouse model of angiogenesis / E. Petitclerc ... [et al.]
  • Analysis of embryonic vascular morphogenesis / T.N. Sato ... [et al.]
  • Epithelial-mesenchymal interactions / B.K. Hall
  • Methods for manipulating the chick limb bud to study gene expression, tissue interactions, and patterning / M.A. Ros ... [et al.]
  • Palate development : in vitro procedures / M.M. Pisano ...[et al.]
  • In vitro fertilization / S. Heyner ... [et al.]
  • Trophoblast differentiation : an in vitro model for trophoblast giant cell development / T.J. Peters ... [et al.]
  • Bone marrow-derived mesenchymal progenitor cells / B. Johnstone ... [et al.]
  • Identification, characterization, and differentiation of human prostate cells / P.P. Mehta ... [et al.]
  • Preparation of chick striated muscle cultures / C. DiLullo ... [et al.]
  • Study of skeletal myogenesis in cultures of unsegmented paraxial mesoderm / A.-G. Borycki ... [et al.]
  • Embryonic limb mesenchyme micromass culture as an in vitro model for chondrogenesis and cartilage maturation / A.M. DeLise ... [et al.]
  • Electroporation-mediated DNA transfection of embryonic chick limb mesenchymal cells / A.M. DeLise ... [et al.]
  • Murine C3H10T1/2 multipotential cells as an in vitro model of mesenchymal chondrogenesis / A.R. Haas ... [et al.]
  • Skeletogenesis : in vitro analysis of bone cell differentiation / A. Majolagde ... [et al.]
  • Studying early hematopoiesis using avian blastoderm cultures / C.A. Eisenberg
  • Isolation and culture of mouse germ cells / M.P. De Miguel ... [et al.]
  • Cadherin-mediated cell-cell interactions / K.A. Knudsen ... [et al.]
  • Analysis of hyaluronan using biotinylated hyaluronan-binding proteins / C.B. Underhill / Microinjection of fluorescently labeled alpha-actinin into living cells / J.M. Sanger ... [et al.]
  • Pax3 and vertebrate development / J.A. Epstein
  • Genetic-engineered models of skeletal diseases I : collagen type X / O. Jacenko
  • Genetic-engineered models of skeletal diseases II : targeting mutations into transgenic mice chondrocytes / S. Garofalo ... [et al.]
  • Transgenic mouse models of craniofacial disorders / Y.-H. Liu ... [et al.]