T cell protocols : development and activation /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2000]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 134. |
| Subjects: |
Table of Contents:
- Pt. 1. Overview: for chapters 2-15
- Insights into T-cell development using transgenic and knockout mice / M. Albert Basson, Rose Zamoyska
- Pt. 2. T cell development
- Isolation and characterization of murine early intrathymic precursor populations / Li Wu, Ken Shortman
- Differentiation of mouse thymocytes in fetal thymus organ culture / Yousuke Takahama
- Purification of immature CD4⁺CD8⁺ thymocytes by panning with anti-CD8 antibody / Terrence G. Gardner, Kelly P. Kearse
- Transfection and transcription of genes in developing thymocytes / Alison M. Michie, Juan Carlos Zúñiga-Pflücker
- Analysis of CD4/CD8 lineage commitment by pronase treatment and reexpression assay / Harumi Suzuki
- Intrathymic delivery of MHC genes using recombinant adenoviruses / Ronald Rooke, Christophe Benoist, Diane Mathis
- Quantitative analysis of the usage of human T-cell receptor α and β chain variable regions by reverse dot-blot hybridization / Takaji Matsutani ... [et al.]
- Flow cytometric detection of cytoplasmic and surface CD3- expression in developing T cells / Karl J. Franek, Robert Chervenak
- Assessing apoptosis of developing T cells by flow cytometry / Caroline D.V.A. Bishop ... [et al.]
- Isolation of T-cell subsets by Magnetic Cell Sorting (MACS) / Luminita A. Stanciu, Ratko Djukanović
- Isolation of T-cell antigens by retrovirus-mediated expression cloning / Toshio Kitamura, Yoshihiro Morikawa
- Flow cytometric analysis of murine T lymphocytes: a practical guide / Melanie S. Vacchio, Elizabeth W. Shores
- Production and characterization of immature murine T-lymphoma cell lines / Ellen R. Richie, Dawn A. Walker
- Production and characterization of T cell hybridomas / Janice White, John Kappler, Philippa Marrack
- Pt. 3. Overview: for chapters 17-29
- Something happens: a brief history of TCR signal transduction / John J. O'Shea
- Pt. 4. T-cell activation
- Measurement of phosphatidylinositol (PI) hydrolysis in activated T lymphocytes / Seth Mark Berney, T. Prescott Atkinson
- Fluorescence polarization as an early measure of T-lymphocyte stimulation / Mordechai Deutsch ... [et al.]
- Measurement of lymphoproliferation at the single-cell level by flow cytometry / Hans Gaines, Gunnel Biberfeld
- Digital image analysis of lymphocyte activation / Bradley W. McIntyre ... [et al.]
- Induction of T-cell signaling by immobilized integrin ligands / Jean Maguire van Seventer, Gijs A. van Seventer
- Cytotoxic T-cell adherence assay (CAA) / Graham R. Leggatt, Jay A. Berzofsky
- Detecting ubiquitinated T-cell antigen receptor subunits by immunoblotting / Allan M. Weissman
- Determination of CD45 tyrosine phosphatase activity in T lymphocytes / David H.W. Ng, Jackie Felberg, Pauline Johnson
- Measurement of protein tyrosine phosphorylation in T-cell subsets by flow cytometry / Dariush Farahi Far, Bernard Rossi
- Biochemical analysis of activated T lymphocytes: protein phosphorylation and Ras, ERK, and JNK activation / Patrick E. Fields, Thomas F. Gajewski
- Activation of heterotrimeric GTP-binding proteins upon TCR/CD3 engagement / Constantine D. Tsoukas, Jack Stanners, Keith A. Ching
- Generation of human T helper 1 and T helper 2 subsets from peripheral blood-derived naive CD4⁺ T cells / Ellen M. Palmer ... [et al.]
- Transcriptional expression analysis of T-cell activation by Multiplex Messenger Assay (MMA) / Karine Bernard ... [et al.].