The molecular biology of insect disease vectors : a methods manual /
| Other Authors: | , , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
London ; New York :
Chapman & Hall,
1997.
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| Edition: | First edition. |
| Subjects: |
Table of Contents:
- Care and maintenance of anopheline mosquito colonies
- Care and maintenance of Aedes mosquito colonies
- Care and maintenance of phlebotomine sandfly colonies
- Care and maintenance of blackfly colonies
- Care and maintenance of tsetse colonies
- Care and maintenance of triatomine colonies
- Infection of mosquitoes with primate malaria
- Infection of mosquitos with filaria
- Infection of phlebotomine sandflies with Leishmania
- Infection of blackflies with Onchocerca
- Infection of tsetse with Trypanosoma brucei rhodesiense and T.b. gambience
- Infection of triatomines with Trypansoma cruzi
- Isolation of genomic DNA
- Isolation and analysis of RNA from insect vectors
- Determination of insect genome size and complexity by reassociation kinetics
- Constructing and screening lambda libraries
- Constructing and screening cosmid libraries
- Constructing and screening cDNA libraries
- Southern/northern blotting and hybridization techniques
- The polymerase chain reaction (PCR) and RT-PCR
- Differential display of mRNA
- Localization of RNA transcripts by in situ hybridization
- In situ localization of proteins in whole mounted tissue
- DNA sequencing and primer extension mapping
- RFLP analysis of insect genomes
- Microsatellite mapping of insect genomes
- Polytene chromosome preparation from anopheline mosquitoes
- In situ hybridization to Anopheles polytene chromosomes
- Microdissection and microamplification of dipteran polytene chromosomes
- RAPD-PCR and SSCP analysis for insect population genetic studies
- PCR amplification of insect ribosomal DNA
- Generation and use of species-specific DNA probes for insect vector identification
- Cloning and detection of insecticide resistance genes
- Microinjection of mosquito embryos
- Transfection of cultured mosquito cells
- Viral transducting vectors for mosquitos
- Viral expression systems and infections in insects
- Establishing primary cell cultures from disease vectors and maintenance of continuous cell lines
- Culture and analysis of insect salivary glands
- Culture and analysis of the insect fat body
- Culture and analysis of insect ovaries
- Culture and analysis of insect midgut
- Isolation and culture of tsetse secondary endosymbionts
- Genetic manipulation of insect symbionts
- PCR-based detection and identification of insect symbionts