Helicobacter pylori protocols /

Bibliographic Details
Other Authors:	Clayton, Christopher L., Mobley, Harry L. T.
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [1997]
Series:	Methods in molecular medicine.
Subjects:	Helicobacter pylori > Laboratory Manuals.

Table of Contents:

Introduction: Medical significance of H. pylori
Detection of H. pylori infection by biopsy urease, histology, and culture
Serology and urea breath test in diagnosis of H. pylori infection
The detection of H. pylori by the polymerase chain reaction
PCR for the detection of H. pylori in gastric juice aspirates and environmental water samples
In Vitro susceptibility testing of H. pylori
Defined media for H. pylori
Studies of respiration in H. pylori
Use of NMR to study H. pylori metabolism
Isolation of H. pylori genomic DNA and restriction analysis
ribosomal RNA gene restriction pattern analysis (ribotyping) of H. pylori
Differentiation of H. pylori strains using PCR RFLP
PCR-DNA sequence typing
H. pylori DNA fingerprinting using the arbitrarily primed PCR (AP-PCR) or random amplified polymorphic DNA (RAPD) method
Molecular methods for detecting ulcerogenic strains of H. pylori
H. pylori DNA transformation by natural competence and electroporation
Random mutagenesis of the H. pylori genome
Construction of the genomic map of H. pylori by pulsed-field gel electrophoresis (PFGE)
Molecular characterization of H. pylori surface antigens
Isolation and characterization of porins from H. pylori
Methods for the identification of H. pylori host receptors
Host responses to H. pylori: molecular analysis of cytokine gene expression
Nonprimate animal models of H. pylori infection
The nonhuman primate model for H. pylori infection