Medical virology : a practical approach /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Oxford ; New York :
IRL Press at Oxford University Press,
[1995]
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| Series: | Practical approach series ;
147. |
| Subjects: |
Table of Contents:
- Traditional techniques of viral diagnosis / C.R. Madeley
- Immunoassays / J.J. Gray and T.G. Wreghitt
- Nucleic acid detection / Jonathan P. Clewley
- Blotting of viral proteins / Sally E. Adams and Nigel R. Burns
- Polymerase chain reaction / P. Simmonds
- Design and testing of antiviral compounds / Derek Kinchington, Hillar Kangro, and Donald J. Jeffries
- Molecular epidemiology / U. Desselberger
- Evolutionary analysis of viruses / Andrew J. Leigh Brown.
- Traditional techniques of viral diagnosis
- Rationale of virus diagnosis
- Detection of virus or viral antigen
- Developments in diagnosis
- Immunoassays
- Enzyme-linked immunosorbent assays
- Radioimmunoassay
- Luminescence assays
- Monoclonal antibodies
- Evaluation of a commercial immunoassay
- Semi-automation and full automation of serological tests
- Quality assurance
- Nucleic acid detection
- Introduction
- Preparation
- Blotting
- Production and purification of probes
- Labelling of probes.
- Hybridization and detection
- Example: parvovirus B19 DNA
- Trouble shooting
- Significance of viral nucleic acid detection
- Blotting of viral proteins
- Introduction
- Isolation of viral proteins
- Blotting
- Applications
- Polymerase chain reaction
- Principles of the polymerase chain reaction
- Sample preparation
- Amplification reactions
- Detection of amplified product
- Applications of PCR
- Design and testing of antiviral compounds
- Introduction
- Assays for virus infectivity
- Toxicity testing in cell culture
- Measurement of compound efficacy in tissue culture
- Activity against isolated viral enzymes
- Molecular epidemiology
- Introduction
- Use of polyacrylamide gel electrophoresis to separate the segments of RNA viruses
- Oligonucleotide maps of RNA virus genomes
- Analysis of restriction endonuclease fragments of DNA virus genomes by PAGE
- Nucleic acid hybridization techniques
- Polymerase chain reactions.
- DNA sequencing
- Monoclonal antibodies
- Virus evolution
- Molecular pathology
- Evolutionary analysis of viruses
- Introduction
- Measures of genetic distance
- Character-based methods
- Statistical significance of tree topologies
- Software for phylogenetic analyses
- Addresses of suppliers.