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In vitro transcription and translation protocols /

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Bibliographic Details
Other Authors: Tymms, Martin J.
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [1995]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 37.
Subjects:
Protein Biosynthesis.
Transcription, Genetic.
Genetic Techniques.
Molecular Biology.
Laboratory Manual.
Table of Contents:
  • Transcription in vitro using bacteriophage RNA polymerases / Elaine T. Schenborn
  • Subtraction hybridization cDNA libraries / Clifford W. Schweinfest ... [et al.]
  • Quantitative measurement of mRNA using the RNase protection assay / Martin J. Tymms
  • Assembly and transcription of chromatin templates using RNA polymerase III / Sara J. Felts
  • Antisense affinity depletion of RNP particles: application to spliceosomal snRNPs / Benjamin J. Blencowe, Silvia M.L. Barabino
  • In vitro mRNA editing using S100 extracts / Jobst Greeve, James Scott.
  • In vitro transcription assay for probing drug-DNA interactions during active transcription of DNA / Don R. Phillips, Donald M. Crothers
  • In vitro reconstitution of progesterone-dependent RNA transcription in nuclear extracts of human breast carcinoma cells / Milan K. Bagchi, Sophia Y. Tsai, Ming-Jer Tsai
  • Plastid in vitro transcription / Kai Tiller, Gerhard Link
  • Microinjection of in vitro transcribed RNA and antisense oligonucleotides in mouse oocytes and early embryos to study the gain- and loss-of-function of genes / Ismail Kola, Sony Heru Sumarsono.
  • mRNA translation in Xenopus oocytes / Aldo Ceriotti, Alan Colman
  • Xenopus egg extract translation system / Glenn M. Matthews, Alan Colman
  • In vitro translation using rabbit reticulocyte lysate / Gregory S. Beckler, David Thompson, Tom Van Oosbree
  • In vitro transcription system from BmN cells of the silkworm, Bombyx mori / Hiroshi Sakurai, Susumu Izumi, Shiro Tomino
  • Translation using a wheat-germ extract / John F. Van Herwynen, Gregory S. Beckler
  • In vitro transcription and translation in a cell-free system from Clostridium tetani / Bettina Andersen-Bechk, Heiner Niemann.
  • Preparation and use of E. coli S-30 extracts / Scott A. Lesley
  • Posttranslational transport and in thylakoido integration of plastid protein / Elisabeth Kruse, Klaus Kloppstech
  • Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro / Ruby H.P. Law, Phillip Nagley
  • Structure-function studies based on in vitro expression / Martin J. Tymms, Paul J. Hertzog
  • [delta]-like plastid transcription factors / Kai Tiller, Gerhard Link
  • Preparation of HeLa nuclear extracts / B. Franklin Pugh.
  • Purification of the human TATA-binding protein, TBP / B. Franklin Pugh
  • DNA-binding studies using in vitro synthesized Myb proteins / Robert George Ramsay
  • DNase I footprinting using PCR-generated end-labeled DNA probes / Rocco C. Iannello
  • Gene isolation by screenig [gamma]gt11 expression libraries with DNA binding-site probes / Bill Kalionis
  • Transcriptional activation analysis by the chloramphenicol acetyl transferase (CAT) enzyme assay / David R. Hodge ... [et al.].