Manipulating the mouse embryo : a laboratory manual /
| Other Authors: | |
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| Format: | Book |
| Language: | English |
| Published: |
Plainview, N.Y. :
Cold Spring Harbor Laboratory Press,
1994.
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| Edition: | Second edition. |
| Subjects: |
Table of Contents:
- Developmental genetics and embryology of the mouse: past, present and future
- Summary of mouse development
- Setting up a colony for the production of transgenic and mutant mice
- Recovery, culture and transfer of embryos and germ cells
- In vitro manipulation of preimplantation embryos
- Production of transgenic mice
- Isolation, culture and manipulation of embryonic stem cells
- Analysis of transgenic mice
- Techniques for visualizing genes, gene products and specialized cell types
- In vitro culture of eggs, embryos, primordial germ cells and teratocarcinoma cells
- Buffers and solutions
- Acidic tyrode solution for removing zonae
- Alkaline phosphatase buffers
- Alkaline phosphatase staining solution
- Avertin, anesthetic
- Bovine serum albumin (BSA)
- Ca⁺⁺/Mg⁺⁺-free PBS
- Ca⁺⁺/Mg⁺⁺-free tyrode ringer's saline (pH 7.6-7.7)
- Hyaluronidase
- NTM buffer (NTMT without tween 20) for in situ hybridization of intact embryos with RNA probes
- NTMT buffer for in situ hybridization of intact embryos with RNA probes
- Pancreatin/trypsin solution for separating germ and tissue layers
- PBSMT for immunohistochemistry of whole mount embryos
- PBT
- Phenol/chloroform solution for isolating total RNA from mouse embryos or fetal tissues
- Phosphate-buffered saline (PBS ̲-- Pronase solution
- Saline/EDTA buffer plus glucose for isolation of germ cells and tissue culture
- 20X SSC
- 20X SSPE
- TE (tris/EDTA) buffer
- 0.25% trypsin in tris-saline for tissue culture
- Trypsin/EDTA solutions
- Sources of information
- Genetic resources
- Books
- Journals
- Databases
- Software
- Suppliers
- Inbred, hybrid and random-bred mice
- Mouse genomic DNAs and RNAs
- Mouse genomic and cDNA libraries and clones
- Equipment and supplies