Methods in nonradioactive detection /

Bibliographic Details
Other Authors:	Howard, Gary C.
Format:	Book
Language:	English
Published:	Norwalk, Conn. : Appleton & Lange, [1993]
Subjects:	DNA > analysis > Laboratory Manuals. Nucleic Acid Hybridization. Proteins > analysis > Laboratory Manuals.

Table of Contents:

Basics of nonradioactive detection systems / Gary C. Howard
General considerations for use of manual / Gary C. Howard
Protein methods
Biotin labeling methods for proteins and carbohydrates / Gary C. Howard
Direct conjugation of enzymes to proteins / Gary C. Howard
Labeling proteins with fluorochromes / Gary C. Howard
Comparing fluorescent organic dyes for biomolecular labeling / Iain D. Johnson, Diane Ryan and Richard P. Haugland
Chemiluminescent detection using alkaline phosphatase: the chemistry / Hashem Akhavan-Tafti, Richard S. Handley and A. Paul Schaap
Detection of proteins on filters by enzymatic methods / Robert Oberfelder
Enzyme-linked immunosorbent assays / J.E. Butler
Automation of protein and immunochemistry methods / Gerald Hahn
DNA methods
Biotin labeling methods for DNA / Jesse Mackey
Direct attachment of enzymes to DNA probes / Jerry L. Ruth
Detection of nucleic acids on filters by enzymatic methods / Jesse Mackey
Polymerase chain reaction and its impact on DNA detection limits / Randall K. Saiki.
(cont) Automated procedures for DNA methods / Emily S. Winn-Deen
Tissue and cell methods
Enzyme-based methods in tissue sections / Richard W. Cartun
In situ hybridization / Jan G.J. Bauman
Chromosome mapping by fluorescent in situ hybridization / Julianne Meyne
Colloidal gold localization of in situ hybrids / Barbara A. Hamkalo, Sandya Narayanswami and Nadja Dvorkin
Immunofluorescence methods for microscopic analysis / Meryl J. Melnicoff
Flow cytometry methods / Bruce D. Jensen and Katharine A. Muirhead.