http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
xvi, 342 pages, 8 unnumbered pages of plates : illustrations (some color) ; 26 cm.
Basics of nonradioactive detection systems / Gary C. Howard -- General considerations for use of manual / Gary C. Howard -- Protein methods -- Biotin labeling methods for proteins and carbohydrates / Gary C. Howard -- Direct conjugation of enzymes to proteins / Gary C. Howard -- Labeling proteins with fluorochromes / Gary C. Howard -- Comparing fluorescent organic dyes for biomolecular labeling / Iain D. Johnson, Diane Ryan and Richard P. Haugland -- Chemiluminescent detection using alkaline phosphatase: the chemistry / Hashem Akhavan-Tafti, Richard S. Handley and A. Paul Schaap -- Detection of proteins on filters by enzymatic methods / Robert Oberfelder -- Enzyme-linked immunosorbent assays / J.E. Butler -- Automation of protein and immunochemistry methods / Gerald Hahn -- DNA methods -- Biotin labeling methods for DNA / Jesse Mackey -- Direct attachment of enzymes to DNA probes / Jerry L. Ruth -- Detection of nucleic acids on filters by enzymatic methods / Jesse Mackey -- Polymerase chain reaction and its impact on DNA detection limits / Randall K. Saiki.
(cont) Automated procedures for DNA methods / Emily S. Winn-Deen -- Tissue and cell methods -- Enzyme-based methods in tissue sections / Richard W. Cartun -- In situ hybridization / Jan G.J. Bauman -- Chromosome mapping by fluorescent in situ hybridization / Julianne Meyne -- Colloidal gold localization of in situ hybrids / Barbara A. Hamkalo, Sandya Narayanswami and Nadja Dvorkin -- Immunofluorescence methods for microscopic analysis / Meryl J. Melnicoff -- Flow cytometry methods / Bruce D. Jensen and Katharine A. Muirhead.
Includes bibliographical references and index.