Nonradioactive labeling and detection of biomolecules /
| Other Authors: | |
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| Format: | Book |
| Language: | English |
| Published: |
Berlin ; New York :
Springer-Verlag,
[1992]
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| Subjects: |
Table of Contents:
- General aspects of nonradioactive labeling and detection
- Standard nonradioactive labeling and detection systems
- Overview of nonradioactive labeling systems
- Digoxigenin: anti-digoxigenin (DIG) system
- Biotin system
- In vivo labeling of DNA probes with 5-BrdU
- Sulfone system
- Colloidal gold as a marker in molecular biology: the use of ultra-small gold probes
- Direct peroxidase labeling of hybridization probes and chemiluminescence detection
- Highly sensitive method for detecting peroxidase in in situ hybridization or immunohistochemical assays
- SNAP system
- Specialized nonradioactive detection systems
- Overview of colorimetric, chemiluminometric, and fluorimetric detection systems
- Colorimetric systems
- Luminescence systems
- Fluorescence systems
- Enhanced systems
- Overview of amplification systems
- Enhanced signal generation by target amplification
- Signal amplification system: substrate cascade
- Application formats
- Overview of application formats
- Blot formats: nucleic acids
- Blot formats: proteins and glycoproteins.
- In situ formats
- Quantitative formats
- Suppliers of reagents
- Suppliers of equipment.